Mycothione reductase explained

mycothione reductase
Ec Number:	1.8.1.15
Go Code:	0050627

In enzymology, a mycothione reductase is an enzyme that catalyzes the chemical reaction

mycothione + NAD(P)H + H ⁺

\rightleftharpoons 2 mycothiol + NADP⁺

in M. tuberculosis and other actinomycetes.

The 2 substrates of this enzyme are mycothiol and NADPH, whereas 2 molecules of mycothione and NADP⁺ are formed as products.

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is mycothiol:NAD(P)+ oxidoreductase. This enzyme is also called mycothiol-disulfide reductase.

References

• Patel MP, Blanchard JS . 1999 . Expression, purification, and characterization of Mycobacterium tuberculosis mycothione reductase . Biochemistry . 38 . 11827 - 33 . 10512639 . 10.1021/bi991025h . 36 .
• Patel MP, Blanchard JS . 2001 . Mycobacterium tuberculosis mycothione reductase: pH dependence of the kinetic parameters and kinetic isotope effects . Biochemistry . 40 . 5119 - 26 . 11318633 . 10.1021/bi0029144 . 17 .