Molybdopterin synthase sulfurtransferase explained

Molybdopterin synthase sulfurtransferase
Ec Number:	2.8.1.11

Molybdopterin synthase sulfurtransferase (adenylyltransferase and sulfurtransferase MOCS3, Cnx5 (gene), molybdopterin synthase sulfurylase) is an enzyme with systematic name persulfurated L-cysteine desulfurase:(molybdopterin-synthase sulfur-carrier protein)-Gly-Gly sulfurtransferase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine

\rightleftharpoons AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + cysteine desulfurase

The enzyme transfers sulfur to form a thiocarboxylate moiety on the C-terminal glycine of the small subunit of molybdopterin synthase.

Notes and References

1. Matthies A, Nimtz M, Leimkühler S . Molybdenum cofactor biosynthesis in humans: identification of a persulfide group in the rhodanese-like domain of MOCS3 by mass spectrometry . Biochemistry . 44 . 21 . 7912–20 . May 2005 . 15910006 . 10.1021/bi0503448 .
2. Leimkühler S, Rajagopalan KV . A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli . The Journal of Biological Chemistry . 276 . 25 . 22024–31 . June 2001 . 11290749 . 10.1074/jbc.M102072200 . free .
3. Hänzelmann P, Dahl JU, Kuper J, Urban A, Müller-Theissen U, Leimkühler S, Schindelin H . Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains . Protein Science . 18 . 12 . 2480–91 . December 2009 . 19798741 . 2821267 . 10.1002/pro.260 .
4. Dahl JU, Urban A, Bolte A, Sriyabhaya P, Donahue JL, Nimtz M, Larson TJ, Leimkühler S . The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli . The Journal of Biological Chemistry . 286 . 41 . 35801–12 . October 2011 . 21856748 . 3195606 . 10.1074/jbc.M111.282368 . free .