Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase explained

Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase
Ec Number:	3.6.1.53

Mn²⁺-dependent ADP-ribose/CDP-alcohol diphosphatase (Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase, ADPRibase-Mn) is an enzyme with systematic name CDP-choline phosphohydrolase.^{[1] [2]} This enzyme catalyses the following chemical reaction

(1) CDP-choline + H₂O

\rightleftharpoons CMP + phosphocholine

(2) ADP-ribose + H₂O

\rightleftharpoons AMP + D-ribose 5-phosphate

This enzyme requires Mn²⁺, which cannot be replaced by Mg²⁺.

Notes and References

1. Canales J, Pinto RM, Costas MJ, Hernández MT, Miró A, Bernet D, Fernández A, Cameselle JC . Rat liver nucleoside diphosphosugar or diphosphoalcohol pyrophosphatases different from nucleotide pyrophosphatase or phosphodiesterase I: substrate specificities of Mg(2+)-and/or Mn(2+)-dependent hydrolases acting on ADP-ribose . Biochimica et Biophysica Acta . 1246 . 2 . 167–77 . January 1995 . 7819284 . 10.1016/0167-4838(94)00191-i .
2. Canales J, Fernández A, Ribeiro JM, Cabezas A, Rodrigues JR, Cameselle JC, Costas MJ . Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase: a novel metallophosphoesterase family preferentially expressed in rodent immune cells . The Biochemical Journal . 413 . 1 . 103–13 . July 2008 . 18352857 . 10.1042/bj20071471 . 26036152 .