| Mittelholstein | |
| Native Name Lang: | de |
| Settlement Type: | Amt |
| Subdivision Type: | Country |
| Subdivision Name: | Germany |
| Subdivision Type2: | State |
| Subdivision Name2: | Schleswig-Holstein |
| Subdivision Type3: | District |
| Subdivision Name3: | Rendsburg-Eckernförde |
| Seat Type: | Region seat |
| Seat: | Hohenwestedt |
| Leader Title: | Amtsvorsteher |
| Leader Name: | Stefan Landt |
| Area Total Km2: | 380,9 |
| Population Total: | 24.029 |
| Population As Of: | 2020-12-31 |
| Website: | www.amt-mittelholstein.de |
Mittelholstein ("central Holstein") is an Amt ("collective municipality") in the district of Rendsburg-Eckernförde, in Schleswig-Holstein, Germany. It was formed on 1 January 2012 from the three former Ämter Aukrug, Hanerau-Hademarschen and Hohenwestedt-Land and the municipality Hohenwestedt. The seat of the Amt is in Hohenwestedt.
The Amt Mittelholstein consists of the following municipalities: