| Winner: | Corinna Tsopei |
| Represented: | Greece |
| Date: | 1 August 1964 |
| Venue: | Miami Beach Convention Hall, Miami Beach, Florida, United States |
| Broadcaster: | CBS |
| Entrants: | 60 |
| Placements: | 15 |
| Congeniality: | Jeanne Venables (California) |
| Photogenic: | Emanuela Stramana (Italy) |
| Best National Costume: | Henny Deul (Holland) |
| Before: | 1963 |
| Next: | 1965 |
Miss Universe 1964 was the 13th Miss Universe pageant, held on 1 August 1964 at the Miami Beach Convention Hall in Miami Beach, Florida, United States. Corinna Tsopei of Greece was crowned by Iêda Maria Vargas of Brazil making her the first and so far only Miss Universe from Greece.
| Placement | Contestant | |
|---|---|---|
| Miss Universe 1964 | ||
| 1st runner-up |
| |
| 2nd runner-up |
| |
| 3rd runner-up |
| |
| 4th runner-up |
| |
| Top 10 | ||
| Top 15 |
|
Sixty contestants competed for the title.
| Country/Territory | Contestant | Age | Hometown | |
|---|---|---|---|---|
 Argentina | María Amalia Ramírez | 19 | Santa Fe | |
| Lidia Lidwina Henriquez | – | Oranjestad | ||
| Ria Lubyen | 22 | Melbourne | ||
 Austria | Gloria Mackh | 22 | Vienna | |
 Bahamas | Catherine Cartwright | 18 | Nassau | |
 Belgium | Danièle Defrere | 20 | Brussels | |
| Olga Mónica del Carpio | 19 | La Paz | ||
 Brazil | Ângela Vasconcelos | 19 | Paraná | |
| Mary Rande Holl | – | Georgetown | ||
 Canada | Mary Lou Farrell | 21 | St. John's | |
| Annette Dona Kulatunga | 19 | Colombo | ||
| Patricia Herrera | 18 | Viña del Mar | ||
 Colombia | Alba Ramírez | 18 | Bogotá | |
 Costa Rica | Dora Marcela Sola | 21 | San José | |
| Iris de Windt | 21 | Willemstad | ||
 Denmark | Yvonne Mortensen | 19 | Copenhagen | |
 Dominican Republic | Clara Chapuseaux | – | Comenador | |
 Ecuador | Tanya Klein | 18 | Guayaquil | |
| Brenda Blackler | 20 | Liverpool | ||
 Finland | Sirpa Wallenius | 21 | Helsinki | |
 France | Edith Noël | 20 | Paris | |
| Marina Kettler | 20 | Berlin | ||
| Corinna Tsopei | 20 | Athens | ||
 Grenada | Christine Hughes | – | St. George's | |
| Henny Deul | 18 | Amsterdam | ||
| Mary Bai | 23 | Hong Kong | ||
 Iceland | Thelma Ingvarsdóttir | 18 | Reykjavík | |
| Meher Castelino | 18 | Bombay | ||
 Ireland | Maurine Lecky | 19 | Dublin | |
 Israel | Ronit Rinat | 18 | Haifa | |
 Italy | Emanuela Stramana | 22 | Venice | |
 Jamaica | Beverly Rerrie | – | Kingston | |
 Japan | Chizuko Matsumoto | 19 | Fukuoka | |
 Luxembourg | Mariette Sophie | – | Luxembourg City | |
| Angela Filmer | 19 | Klang | ||
 New Zealand | Lyndal Ursula Cruickshank | 21 | Auckland | |
| Edna Park | 19 | Lagos | ||
 Norway | Jorunn Barun | 18 | Oslo | |
| Okinawa | Toyoko Uehara | 20 | Okinawa | |
| Maritza Montilla | – | Coclé | ||
 Paraguay | Miriam Riart Brugada | 21 | Asunción | |
 Peru | Miluska Vondrak | 20 | Loreto | |
 Philippines | Myrna Panlilio | 22 | Manila | |
 Puerto Rico | Yolanda Rodríguez | 21 | Rio Piedras | |
| Lana Yu Yi | 20 | Taipei | ||
| Stella Hadley | 18 | Kingstown | ||
| Wendy Barrie | 19 | Glasgow | ||
 South Africa | Gail Robinson | 20 | Johannesburg | |
| Shin Jung-hyun | 21 | Seoul | ||
 Spain | Maria José Ulloa Madronero | 19 | Madrid | |
| Cynthia Ingrid Dijkstra | 18 | Paramaribo | ||
 Sweden | Sivi Aaberg | 22 | Stockholm | |
 Switzerland | Sandra Sulser | – | Lausanne | |
| Julia Merlene Laurence | – | Port of Spain | ||
| Claudine Younes | 22 | Tunis | ||
 Turkey | Inçi Duran | 18 | Istanbul | |
 United States | Bobbi Johnson[1] | 19 | Washington D.C. | |
 Uruguay | Delia Babiak | – | Montevideo | |
| Mercedes Revenga | 18 | Caracas | ||
| Marilyn Joy Samuel | 22 | Monmouthsire |
Last competed in 1952:
Last competed in 1954:
Last competed in 1958:
Last competed in 1960:
Last competed in 1961:
Last competed in 1962:
Saint Vincent and the Grenadines