| Winner: | Gilda Jovine |
| Represented: | Constanza |
| Date: | March 25, 2000 |
| Venue: | Renaissance Auditorio de Festival del Hotel Jaragua, Santo Domingo, Dominican Republic |
| Next Pageant: | Miss Universe 2000 |
| Broadcaster: | Telemicro |
| Entrants: | 54 |
Miss Universe Dominicana 2000 was held on March 25, 2000. There were 54 candidates, representing provinces and municipalities, who entered. The winner would represent the Dominican Republic at Miss Universe 2000. The Miss Mundo Dominicana would enter Miss World 2000. The Miss Internacional Dominicana would enter in Miss International 2000. The first runner-up would enter in Miss Tropical International 2000. The second runner-up would enter in Reinado Internacional del Café 2000. The rest of finalists entered different pageants. This edition would be the last edition to select Top 20 quarter finalist in the history of Miss Dominican Republic.
| Final Results | Contestant | |
|---|---|---|
| Miss Universe Dominicana 2000 | ||
| Miss Mundo Dominicana |
| |
| Miss Internacional Dominicana |
| |
| 1st Runner-up |
| |
| 2nd Runner-up |
| |
| Semi-finalists |
| |
| Quarter-finalists |
|
| Represented | Contestant | Age | Height | Hometown | |
|---|---|---|---|---|---|
| Clarisa de Lara Merán | 20 | Santo Domingo | |||
| Airiz Mella Escandón | 22 | Baní | |||
| Anna María Torres Bosch | 19 | Santo Domingo | |||
| Victoria María Reyes Báez | 18 | Santo Domingo | |||
| Mariely Bisonó Moncada | 23 | Bonao | |||
| Tania Lara Medina | 25 | San Francisco de Macorís | |||
| Lariela Rodríguez Landro | 19 | Brooklyn | |||
| Oscarina Crisóstomo de la Rosa | 22 | Orlando | |||
| Gilda Evangelina Jovine Gross | 19 | Santo Domingo | |||
| Carolina Oviedo Albuquerque | 21 | Santo Domingo | |||
| Arianna Campusano Reyonsa | 20 | Santiago de los Caballeros | |||
| Odette Carillo López | 24 | Santiago de los Caballeros | |||
| Claudia Santaella Quiñónez | 19 | Santo Domingo | |||
| Clary Karina Tineo García | 19 | San Francisco de Macorís | |||
| Viviana Sánchez Polanco | 26 | Santiago de los Caballeros | |||
| Raiza Colmenares Valencia | 18 | Santiago de los Caballeros | |||
| Alecia Moreno Taradeos | 20 | Santo Domingo | |||
| Paola Gutiérrez de Camacho | 24 | Moca | |||
| Samantha Cáceres de los Santos | 19 | Villa Hermosa | |||
| Eugenia Vallamón Rosario | 18 | Santiago de los Caballeros | |||
| Suly Marleny de la Mota Rosario | 18 | Imbert | |||
| Andrea Peña de los Santos | 21 | Santiago de los Caballeros | |||
| Mónica del Carmen Abreu Matos | 25 | Jarabacoa | |||
| Carolina Gómez Pichardo | 24 | Bonao | |||
| Laurette García Rodríguez | 18 | La Romana | |||
| Teresa Fernández Inoa | 22 | La Romana | |||
| Nathaly Durán Escamoso | 21 | Santiago de los Caballeros | |||
| Jeudy María Melo Espinal | 23 | Santiago de los Caballeros | |||
| Alma Santana Reyes | 19 | Santo Domingo | |||
| Yaneida Elizabeth Polanco Brito | 21 | Santiago de los Caballeros | |||
| Silvana Andreina Santos Mateo | 22 | Santo Domingo | |||
| Yailitza Sarita Palacios | 19 | Santo Domingo | |||
| Landy Hernández Bautista | 20 | Nagua | |||
| Laura Fermín Castañeda | 25 | Baní | |||
| Ana María Veaux Rojas | 24 | Santo Domingo | |||
| Carina Pérez Polanco | 20 | Santo Domingo | |||
| Mía Tatiana Sued Alcántara | 21 | Santo Domingo | |||
| Eva María Ynoa Tavares | 20 | San Francisco de Macorís | |||
| Marlene Raydirys Burgos Mota | 23 | San Felipe de Puerto Plata | |||
| Mayline Rosario Veras | 23 | Salcedo | |||
| Daniela Andrade Espaillat | 18 | Las Terrenas | |||
| Sarah Carolina Medina de la Rosa | 21 | San Cristóbal | |||
| Dulce Esperanza Tavarez Ynoa | 22 | San Francisco de Macorís | |||
| Reimy Solano Torres | 18 | Santiago de los Caballeros | |||
| Arianna María Sosa Pineda | 20 | Santo Domingo | |||
| Yaira María Abreu González | 18 | Santiago de los Caballeros | |||
| Yady Josefina Rosado Oviedo | 18 | Santiago de los Caballeros | |||
| Olivia del Carmen Arredondo Cid | 21 | Santo Domingo | |||
| Alba Ynoa Medina | 25 | Sosúa | |||
| Paula Roman Celis | 24 | Santo Domingo | |||
| Marilyn Mena Mejía | 20 | Santo Domingo | |||
| Antonieta Reyes Estevez | 24 | Santiago de los Caballeros | |||
| Araiza Peña Montesdeoca | 19 | Santiago de los Caballeros | |||
| Cristina Miverva Cruz Mirabal | 22 | Santo Domingo |