| Winner: | Clara Andrea Herrera Vallamon |
| Represented: | Sánchez Ramírez |
| Date: | February 20, 1965 |
| Venue: | La Concha Acústica del Hotel Embajador, Santo Domingo, Dominican Republic |
| Next Pageant: | Miss Universe 1965 |
| Returns: | Ciudad Santo Domingo |
| Broadcaster: | Color Vision |
| Entrants: | 26 |
The Señorita República Dominicana 1965 pageant took place on February 20, 1965, featuring a field of 26 contestants vying for the national crown. The victor would subsequently represent the Dominican Republic at the Miss Universe 1965 competition. The pageant included participants from all 25 provinces and one municipality.
During the event, the top 10 contestants showcased their evening gowns and responded to questions, a segment designed to determine the finalists for the subsequent round. The ensuing top 5 finalists then faced additional questions, contributing to the ultimate selection of the pageant winner.