Mir-30 microRNA precursor explained
| mir-30 microRNA precursor |
| Symbol: | mir-30 |
| Rfam: | RF00131 |
| Mirbase: | MI0000088 |
| Mirbase Family: | MIPF0000005 |
| Rna Type: | Gene
- miRNA
|
| Tax Domain: | Eukaryota |
miR-30 microRNA precursor is a small non-coding RNA that regulates gene expression. Animal microRNAs are transcribed as pri-miRNA (primary miRNA) of varying length which in turns are processed in the nucleus by Drosha into ~70 nucleotide stem-loop precursor called pre-miRNA (precursor miRNA) and subsequently processed by the Dicer enzyme to give a mature ~22 nucleotide product. In this case the mature sequence comes from both the 3' (miR-30)[1] and 5' (mir-97-6)[2] arms of the precursor. The products are thought to have regulatory roles through complementarity to mRNA.[3]
A screen of 17 miRNAs that have been predicted to regulate a number of breast cancer associated genes found variations in the microRNAsmiR-17 and miR-30c-1, these patients were noncarriers of BRCA1 or BRCA2 mutations, lending the possibility that familial breast cancer may be caused by variation in these miRNAs.[4]
Members of the miR-30 family have been found to be highly expressed in heart cells.[5]
Targets of miR-30
It has been shown that the integrin ITGB3 and the ubiquitin conjugating E2 enzyme UBC9 are downregulated by miR-30.[6] It has also been suggested that the TP53 protein may be a target of miR-30c and miR-30e. An immunoblot analysis revealed that p53 expression levels were elevated upon knockdown of miR-30c and miR-30e.[7]
Further reading
- Agrawal R, Tran U, Wessely O . The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1 . Development . 136 . 23 . 3927–36 . December 2009 . 19906860 . 2778741 . 10.1242/dev.037432 .
- Su S, Shao J, Zhao Q, etal . MiR-30b Attenuates Neuropathic Pain by Regulating Voltage-Gated Sodium Channel Nav1.3 in Rats . Front Mol Neurosci . 10 . 126 . May 2017 . 28529474 . 5418349 . 10.3389/fnmol.2017.00126 . free .
Notes and References
- Lagos-Quintana . M . Rauhut R . Lendeckel W . Tuschl T . 2001 . Identification of novel genes coding for small expressed RNAs . Science . 294 . 853 - 858 . 11679670 . 10.1126/science.1064921 . 5543. 2001Sci...294..853L . 11858/00-001M-0000-0012-F65F-2 . 18101169 . free .
- Mourelatos . Z . Dostie J . Paushkin S . Sharma A . Charroux B . Abel L . Rappsilber J . Juri Rappsilber . Mann M . Dreyfuss G . 2002 . miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs . Genes Dev . 16 . 720 - 728 . 11914277 . 10.1101/gad.974702 . 6 . 155365.
- Ambros V . microRNAs: tiny regulators with great potential . Cell . 107 . 7 . 823–6 . 2001 . 11779458 . 10.1016/S0092-8674(01)00616-X. free .
- Shen J, Ambrosone CB, Zhao H . Novel genetic variants in microRNA genes and familial breast cancer. Int J Cancer . 124 . 5 . 1178–82 . 2009 . 19048628 . 10.1002/ijc.24008. free .
- Duisters RF, Tijsen AJ, Schroen B, etal . miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial matrix remodeling . Circ. Res. . 104 . 2 . 170–8, 6p following 178 . January 2009 . 19096030 . 10.1161/CIRCRESAHA.108.182535 .
- Yu F, Deng H, Yao H, Liu Q, Su F, Song E . Mir-30 reduction maintains self-renewal and inhibits apoptosis in breast tumor-initiating cells . Oncogene . 29 . 29 . 4194–204 . July 2010 . 20498642 . 10.1038/onc.2010.167 .
- Li J, Donath S, Li Y, Qin D, Prabhakar BS, Li P . McManus . Michael T. . miR-30 regulates mitochondrial fission through targeting p53 and the dynamin-related protein-1 pathway . PLOS Genet. . 6 . 1 . e1000795 . January 2010 . 20062521 . 2793031 . 10.1371/journal.pgen.1000795 . free .
