Mir-145 Explained

mir-145
Symbol:	mir-145
Rfam:	RF00675
Mirbase Family:	MIPF0000079
Rna Type:	microRNA
Tax Domain:	Eukaryota

In molecular biology, mir-145 microRNA is a short RNA molecule that in humans is encoded by the MIR145 gene. MicroRNAs function to regulate the expression levels of other genes by several mechanisms.^[1]

Targets

MicroRNAs are involved in down-regulation of a variety of target genes. Götte et al. have shown that experimental over-expression of mir-145 down-regulates the junctional cell adhesion molecule JAM-A as well as the actin bundling protein fascin in breast cancer and endometriosis cells, resulting in a reduction of cell motility.^{[2] [3]} Larsson et al.^[4] showed that miR-145 targets the 3' UTR of the FLI1 gene, a finding that was later supported by Zhang et al.

Role in cancer

miR-145 is hypothesised to be a tumor suppressor.^[5] miR-145 has been shown to be down-regulated in breast cancer.^[3] miR-145 is also involved in colon cancer ^{[6] [7] [8]} and acute myeloid leukemia.^[9]

References

Further reading

• Zhang J, Guo H, Qian G, Ge S, Ji H, Hu X, Chen W . MiR-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic network . Molecular Cancer . 9 . 211 . 2010 . 20687965 . 2924312 . 10.1186/1476-4598-9-211 . free .
• Zaman MS, Chen Y, Deng G, Shahryari V, Suh SO, Saini S, Majid S, Liu J, Khatri G, Tanaka Y, Dahiya R . The functional significance of microRNA-145 in prostate cancer . British Journal of Cancer . 103 . 2 . 256–64 . Jul 2010 . 20588276 . 2906737 . 10.1038/sj.bjc.6605742 .
• Sachdeva M, Mo YY . miR-145-mediated suppression of cell growth, invasion and metastasis . American Journal of Translational Research . 2 . 2 . 170–80 . 2010 . 20407606 . 2855636 .
• Kano M, Seki N, Kikkawa N, Fujimura L, Hoshino I, Akutsu Y, Chiyomaru T, Enokida H, Nakagawa M, Matsubara H . miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma . International Journal of Cancer . 127 . 12 . 2804–14 . Dec 2010 . 21351259 . 10.1002/ijc.25284 . 25480039 . free .
• Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, Nishiyama K, Fujimura L, Kikkawa N, Seki N, Nakagawa M . miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer . British Journal of Cancer . 102 . 5 . 883–91 . Mar 2010 . 20160723 . 2833258 . 10.1038/sj.bjc.6605570 .
• Gregersen LH, Jacobsen AB, Frankel LB, Wen J, Krogh A, Lund AH . MicroRNA-145 targets YES and STAT1 in colon cancer cells . PLOS ONE . 5 . 1 . e8836 . 2010 . 20098684 . 2809101 . 10.1371/journal.pone.0008836 . Kudla . Grzegorz . free . 2010PLoSO...5.8836G .
• Sachdeva M, Mo YY . MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1 . Cancer Research . 70 . 1 . 378–87 . Jan 2010 . 19996288 . 2805032 . 10.1158/0008-5472.CAN-09-2021 .
• Larsson E, Fredlund Fuchs P, Heldin J, Barkefors I, Bondjers C, Genové G, Arrondel C, Gerwins P, Kurschat C, Schermer B, Benzing T, Harvey SJ, Kreuger J, Lindahl P . Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1 . Genome Medicine . 1 . 11 . 108 . 2009 . 19917099 . 2808743 . 10.1186/gm108 . free .
• Ostenfeld MS, Bramsen JB, Lamy P, Villadsen SB, Fristrup N, Sørensen KD, Ulhøi B, Borre M, Kjems J, Dyrskjøt L, Orntoft TF . miR-145 induces caspase-dependent and -independent cell death in urothelial cancer cell lines with targeting of an expression signature present in Ta bladder tumors . Oncogene . 29 . 7 . 1073–84 . Feb 2010 . 19915607 . 10.1038/onc.2009.395 . 25309570 .
• Starczynowski DT, Kuchenbauer F, Argiropoulos B, Sung S, Morin R, Muranyi A, Hirst M, Hogge D, Marra M, Wells RA, Buckstein R, Lam W, Humphries RK, Karsan A . Identification of miR-145 and miR-146a as mediators of the 5q- syndrome phenotype . Nature Medicine . 16 . 1 . 49–58 . Jan 2010 . 19898489 . 10.1038/nm.2054 . 7987486 .
• Arndt GM, Dossey L, Cullen LM, Lai A, Druker R, Eisbacher M, Zhang C, Tran N, Fan H, Retzlaff K, Bittner A, Raponi M . Characterization of global microRNA expression reveals oncogenic potential of miR-145 in metastatic colorectal cancer . BMC Cancer . 9 . 374 . 2009 . 19843336 . 2770572 . 10.1186/1471-2407-9-374 . free .
• Zhang C . MicroRNA-145 in vascular smooth muscle cell biology: a new therapeutic target for vascular disease . Cell Cycle . 8 . 21 . 3469–73 . Nov 2009 . 19829088 . 10.4161/cc.8.21.9837 . 3593963 .
• Zeng L, Carter AD, Childs SJ . miR-145 directs intestinal maturation in zebrafish . Proceedings of the National Academy of Sciences of the United States of America . 106 . 42 . 17793–8 . Oct 2009 . 19805048 . 2764920 . 10.1073/pnas.0903693106 . free . 2009PNAS..10617793Z .
• Spizzo R, Nicoloso MS, Lupini L, Lu Y, Fogarty J, Rossi S, Zagatti B, Fabbri M, Veronese A, Liu X, Davuluri R, Croce CM, Mills G, Negrini M, Calin GA . miR-145 participates with TP53 in a death-promoting regulatory loop and targets estrogen receptor-alpha in human breast cancer cells . Cell Death and Differentiation . 17 . 2 . 246–54 . Feb 2010 . 19730444 . 10.1038/cdd.2009.117 . 3648637 .
• Xin M, Small EM, Sutherland LB, Qi X, McAnally J, Plato CF, Richardson JA, Bassel-Duby R, Olson EN . MicroRNAs miR-143 and miR-145 modulate cytoskeletal dynamics and responsiveness of smooth muscle cells to injury . Genes & Development . 23 . 18 . 2166–78 . Sep 2009 . 19720868 . 2751981 . 10.1101/gad.1842409 .
• Cordes KR, Sheehy NT, White MP, Berry EC, Morton SU, Muth AN, Lee TH, Miano JM, Ivey KN, Srivastava D . miR-145 and miR-143 regulate smooth muscle cell fate and plasticity . Nature . 460 . 7256 . 705–10 . Aug 2009 . 19578358 . 2769203 . 10.1038/nature08195 . 2009Natur.460..705C .
• Cheng Y, Liu X, Yang J, Lin Y, Xu DZ, Lu Q, Deitch EA, Huo Y, Delphin ES, Zhang C . MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation . Circulation Research . 105 . 2 . 158–66 . Jul 2009 . 19542014 . 2728297 . 10.1161/CIRCRESAHA.109.197517 .
• Cho WC, Chow AS, Au JS . Restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor mutation . European Journal of Cancer . 45 . 12 . 2197–206 . Aug 2009 . 19493678 . 10.1016/j.ejca.2009.04.039 .
• Chivukula RR, Mendell JT . Abate and switch: miR-145 in stem cell differentiation . Cell . 137 . 4 . 606–8 . May 2009 . 19450510 . 10.1016/j.cell.2009.04.059 . free .
• Xu N, Papagiannakopoulos T, Pan G, Thomson JA, Kosik KS . MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells . Cell . 137 . 4 . 647–58 . May 2009 . 19409607 . 10.1016/j.cell.2009.02.038 . 18601406 . free .
• La Rocca G, Badin M, Shi B, Xu SQ, Deangelis T, Sepp-Lorenzinoi L, Baserga R . Mechanism of growth inhibition by MicroRNA 145: the role of the IGF-I receptor signaling pathway . Journal of Cellular Physiology . 220 . 2 . 485–91 . Aug 2009 . 19391107 . 10.1002/jcp.21796 . 44343336 .
• Wang S, Bian C, Yang Z, Bo Y, Li J, Zeng L, Zhou H, Zhao RC . miR-145 inhibits breast cancer cell growth through RTKN . International Journal of Oncology . 34 . 5 . 1461–6 . May 2009 . 19360360 . 10.3892/ijo_00000275 . free .
• Zheng L, Pu J, Qi T, Qi M, Li D, Xiang X, Huang K, Tong Q . miRNA-145 targets v-ets erythroblastosis virus E26 oncogene homolog 1 to suppress the invasion, metastasis, and angiogenesis of gastric cancer cells . Molecular Cancer Research . 11 . 2 . 182–93 . Feb 2013 . 23233482 . 10.1158/1541-7786.MCR-12-0534 . free .

Notes and References

1. Web site: Entrez Gene: MicroRNA 145. 2015-01-26 .
2. Adammek . Marlene . MicroRNA miR-145 inhibits proliferation, invasiveness, and stem cell phenotype of an in vitro endometriosis model by targeting multiple cytoskeletal elements and pluripotency factors. . Fertility and Sterility . 2013 . 99 . 5 . 1346–1355.e5 . 10.1016/j.fertnstert.2012.11.055 . 23312222. free .
3. Götte M, Mohr C, Koo CY, Stock C, Vaske AK, Viola M, Ibrahim SA, Peddibhotla S, Teng YH, Low JY, Ebnet K, Kiesel L, Yip GW . miR-145-dependent targeting of junctional adhesion molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness . Oncogene . 29 . 50 . 6569–80 . Dec 2010 . 20818426 . 10.1038/onc.2010.386 . 11455884 .
4. Larsson E, Fredlund Fuchs P, Heldin J, Barkefors I, Bondjers C, Genové G, Arrondel C, Gerwins P, Kurschat C, Schermer B, Benzing T, Harvey SJ, Kreuger J, Lindahl P . Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1 . Genome Medicine . 1 . 11 . 108 . 2009 . 19917099 . 2808743 . 10.1186/gm108 . free .
5. Sachdeva M, Zhu S, Wu F, Wu H, Walia V, Kumar S, Elble R, Watabe K, Mo YY . p53 represses c-Myc through induction of the tumor suppressor miR-145 . Proceedings of the National Academy of Sciences of the United States of America . 106 . 9 . 3207–12 . Mar 2009 . 19202062 . 2651330 . 10.1073/pnas.0808042106 . free . 2009PNAS..106.3207S .
6. Zhang J, Guo H, Zhang H, Wang H, Qian G, Fan X, Hoffman AR, Hu JF, Ge S . Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene . Cancer . 117 . 1 . 86–95 . Jan 2011 . 20737575 . 2995010 . 10.1002/cncr.25522 .
7. Slaby O, Svoboda M, Fabian P, Smerdova T, Knoflickova D, Bednarikova M, Nenutil R, Vyzula R . Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer . Oncology . 72 . 5–6 . 397–402 . 2007 . 18196926 . 10.1159/000113489 . 207615720 .
8. Mazza. Tommaso. Mazzoccoli. Gianluigi. Fusilli. Caterina. Capocefalo. Daniele. Panza. Anna. Biagini. Tommaso. Castellana. Stefano. Gentile. Annamaria. De Cata. Angelo. 2016-05-19. Multifaceted enrichment analysis of RNA-RNA crosstalk reveals cooperating micro-societies in human colorectal cancer. Nucleic Acids Research. 44. 9. 4025–4036. 10.1093/nar/gkw245. 1362-4962. 4872111. 27067546.
9. Starczynowski DT, Morin R, McPherson A, Lam J, Chari R, Wegrzyn J, Kuchenbauer F, Hirst M, Tohyama K, Humphries RK, Lam WL, Marra M, Karsan A . Genome-wide identification of human microRNAs located in leukemia-associated genomic alterations . Blood . 117 . 2 . 595–607 . Jan 2011 . 20962326 . 10.1182/blood-2010-03-277012 . free .