| Official Name: | Middlebrook, Missouri |
| Settlement Type: | Unincorporated community |
| Subdivision Type: | Country |
| Subdivision Name: | United States |
| Subdivision Type1: | State |
| Subdivision Name1: | Missouri |
| Subdivision Type2: | County |
| Subdivision Name2: | Iron, St. Francois |
| Timezone: | Central (CST) |
| Utc Offset: | -6 |
| Timezone Dst: | CDT |
| Utc Offset Dst: | -5 |
| Elevation Ft: | 1132 |
| Coordinates: | 37.6628°N -90.6483°W |
| Area Code: | 573 |
| Blank Name: | GNIS feature ID |
| Blank Info: | 756387 |
Middlebrook is an unincorporated community on the Iron County/St. Francois County line in the U.S. state of Missouri. It is located approximately six miles north of Ironton, four miles east of Belleview, and seven miles southeast of Belgrade and Caledonia.
Middlebrook was laid out in 1856.[1] A post office called Middlebrook was established in 1858.[2]