| Middle Brook | |
| Pushpin Map: | New York |
| Pushpin Map Caption: | Location of the mouth of Middle Brook |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | New York |
| Subdivision Type4: | County |
| Subdivision Name4: | Delaware |
| Source1 Coordinates: | 42.4898°N -74.5979°W |
| Mouth: | Charlotte Creek |
| Mouth Coordinates: | 42.4856°N -74.8235°W[1] |
| Mouth Elevation: | 1260feet |
| Tributaries Left: | Center Brook |
| Tributaries Right: | Tedle Brook |
Middle Brook is a river in Delaware County, New York and Schoharie County, New York. It flows into Charlotte Creek northeast of Davenport.