| Official Name: | Middle Assendon |
| Coordinates: | 51.564°N -0.937°W |
| Os Grid Reference: | SU7385 |
| Label Position: | left |
| Civil Parish: | Bix and Assendon |
| Shire District: | South Oxfordshire |
| Shire County: | Oxfordshire |
| Region: | South East England |
| Country: | England |
| Constituency Westminster: | Henley |
| Post Town: | Henley-on-Thames |
| Postcode District: | RG9 |
| Postcode Area: | RG |
| Dial Code: | 01491 |
| Website: | Bix and Assendon website |
Middle Assendon is a village in the Stonor valley in the Chiltern Hills. It is about 2miles northwest of Henley-on-Thames in South Oxfordshire, England. The village has a public house, the Rainbow Inn.