MicroRNA 148a explained

MicroRNA 148a is a miRNA that in humans is encoded by the MIR148A gene.^[1]

Function

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding.

The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products.

The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop.

Further reading

• Fujita Y, Kojima K, Ohhashi R, Hamada N, Nozawa Y, Kitamoto A, Sato A, Kondo S, Kojima T, Deguchi T, Ito M . MiR-148a attenuates paclitaxel resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression . J. Biol. Chem. . 285 . 25 . 19076–84 . 2010 . 20406806 . 2885186 . 10.1074/jbc.M109.079525 . free .
• Hummel R, Hussey DJ, Michael MZ, Haier J, Bruewer M, Senninger N, Watson DI . MiRNAs and their association with locoregional staging and survival following surgery for esophageal carcinoma . Ann. Surg. Oncol. . 18 . 1 . 253–60 . 2011 . 20628822 . 10.1245/s10434-010-1213-y . 26439245 .
• Haflidadóttir BS, Bergsteinsdóttir K, Praetorius C, Steingrímsson E . miR-148 regulates Mitf in melanoma cells . PLOS ONE . 5 . 7 . e11574 . 2010 . 20644734 . 2904378 . 10.1371/journal.pone.0011574 . 2010PLoSO...511574H . free .
• Murata T, Takayama K, Katayama S, Urano T, Horie-Inoue K, Ikeda K, Takahashi S, Kawazu C, Hasegawa A, Ouchi Y, Homma Y, Hayashizaki Y, Inoue S . miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression . Prostate Cancer Prostatic Dis. . 13 . 4 . 356–61 . 2010 . 20820187 . 10.1038/pcan.2010.32 .
• Giraud-Triboult K, Rochon-Beaucourt C, Nissan X, Champon B, Aubert S, Piétu G . Combined mRNA and microRNA profiling reveals that miR-148a and miR-20b control human mesenchymal stem cell phenotype via EPAS1 . Physiol. Genomics . 43 . 2 . 77–86 . 2011 . 21081659 . 10.1152/physiolgenomics.00077.2010 .
• Muiños-Gimeno M, Espinosa-Parrilla Y, Guidi M, Kagerbauer B, Sipilä T, Maron E, Pettai K, Kananen L, Navinés R, Martín-Santos R, Gratacòs M, Metspalu A, Hovatta I, Estivill X . Human microRNAs miR-22, miR-138-2, miR-148a, and miR-488 are associated with panic disorder and regulate several anxiety candidate genes and related pathways . Biol. Psychiatry . 69 . 6 . 526–33 . 2011 . 21168126 . 10.1016/j.biopsych.2010.10.010 . 206101163 . free .
• Pavicic W, Perkiö E, Kaur S, Peltomäki P . Altered methylation at microRNA-associated CpG islands in hereditary and sporadic carcinomas: a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach . Mol. Med. . 17 . 7–8 . 726–35 . 2011 . 21327300 . 3146601 . 10.2119/molmed.2010.00239 .
• Zhang H, Li Y, Huang Q, Ren X, Hu H, Sheng H, Lai M . MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer . Cell Death Differ. . 18 . 11 . 1702–10 . 2011 . 21455217 . 3190106 . 10.1038/cdd.2011.28 .
• Kulkarni S, Savan R, Qi Y, Gao X, Yuki Y, Bass SE, Martin MP, Hunt P, Deeks SG, Telenti A, Pereyra F, Goldstein D, Wolinsky S, Walker B, Young HA, Carrington M . Differential microRNA regulation of HLA-C expression and its association with HIV control . Nature . 472 . 7344 . 495–8 . 2011 . 21499264 . 3084326 . 10.1038/nature09914 . 2011Natur.472..495K .

Notes and References

1. Web site: Entrez Gene: MicroRNA 148a. 2017-09-08.