Methylenetetrahydrofolate dehydrogenase (NAD+) explained

methylenetetrahydrofolate dehydrogenase (NAD^+)
Ec Number:	1.5.1.15
Cas Number:	82062-90-6
Go Code:	0004487

In enzymology, a methylenetetrahydrofolate dehydrogenase (NAD⁺) is an enzyme that catalyzes a chemical reaction.^[1]

5,10-methylenetetrahydrofolate + NAD⁺

\rightleftharpoons 5,10-methenyltetrahydrofolate + NADH + H⁺

Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NAD⁺, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NAD⁺ oxidoreductase. This enzyme is also called methylenetetrahydrofolate dehydrogenase (NAD⁺). This enzyme participates in one carbon pool by folate.^[2]

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .^[3]

References

• Moore MR, O'Brien WE, Ljungdahl LG . 1974 . Purification and characterization of nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum . J. Biol. Chem. . 249 . 5250 - 3 . 4153026 . 16 .

Notes and References

1. Web site: 1.5.1.15: methylenetetrahydrofolate dehydrogenase (NAD+) - BRENDA Enzyme Database . 2023-02-18 . www.brenda-enzymes.org.
2. Web site: Methylenetetrahydrofolate dehydrogenase NAD . 2023-02-18 . LOINC . en-US.
3. Web site: Bank . RCSB Protein Data . RCSB PDB - 1CKM: STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP . 2023-02-18 . www.rcsb.org . en-US.