sRNA162, sRNA154, sRNA41 are small non-coding RNA (sRNA) identified together with 248 other sRNA candidates by RNA sequencing in methanogenic archaeon Methanosarcina mazei Gö1.[1] These sRNAs were further characterised. It was shown that sRNA162 can interact with both, a cis- and a trans-encoded mRNAs using two distinct domains. The sRNA overlaps the 5′UTR of the MM2442 mRNA and acts as a cis-encoded antisense RNA, and it also regulates MM2441 expression as a trans-encoded sRNA. It exhibits a regulatory role in the metabolic switch between methanol and trimethylamine as carbon and energy source. [2] sRNA154, exclusively expressed under nitrogen deficiency, has a central regulatory role in nitrogen metabolism affecting nitrogenase and glutamine synthetase by masking the ribosome binding site or positively affecting transcript stability. [3] sRNA41, highly expressed during nitrogen sufficiency, is capable to bind several ribosome binding sites independently within a polycistronic mRNA. It was proposed to inhibits translation initiation of all ACDS (acetyl-CoA decarbonylase/synthase complex) genes in N-dependent manner. [4]
Bacterial sRNA involved in nitrogen metabolism: NsiR4
Other archaeal sRNAs: