Methanol dehydrogenase (cytochrome c) explained

Methanol dehydrogenase (cytochrome c)
Ec Number:	1.1.2.7
Cas Number:	37205-43-9
Width:	270

Methanol dehydrogenase (cytochrome c) (methanol dehydrogenase, MDH) is an enzyme with systematic name methanol:cytochrome c oxidoreductase.^{[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]} This enzyme catalyses the following chemical reaction

a primary alcohol + 2 ferricytochrome cL

\rightleftharpoons an aldehyde + 2 ferrocytochrome cL + 2 H⁺

A periplasmic quinoprotein alcohol dehydrogenase is only present in methylotrophic bacteria.

Notes and References

1. Anthony C, Zatman LJ . The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27 . The Biochemical Journal . 92 . 3 . 614–21 . September 1964 . 10.1042/bj0920614 . 4378696 . 1206111 .
2. Anthony C, Zatman LJ . The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group . The Biochemical Journal . 104 . 3 . 960–9 . September 1967 . 10.1042/bj1040960 . 6049934 . 1271238 .
3. Duine JA, Frank J, Verwiel PE . Structure and activity of the prosthetic group of methanol dehydrogenase . European Journal of Biochemistry . 108 . 1 . 187–92 . 1980 . 6250827 . 10.1111/j.1432-1033.1980.tb04711.x . free .
4. Salisbury SA, Forrest HS, Cruse WB, Kennard O . A novel coenzyme from bacterial primary alcohol dehydrogenases . Nature . 280 . 5725 . 843–4 . August 1979 . 471057 . 10.1038/280843a0 .
5. Cox JM, Day DJ, Anthony C . The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria . Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology . 1119 . 1 . 97–106 . February 1992 . 1311606 . 10.1016/0167-4838(92)90240-E .
6. Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C . The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues . Nature Structural Biology . 1 . 2 . 102–5 . February 1994 . 7656012 . 10.1038/nsb0294-102 .
7. Xia ZX, He YN, Dai WW, White SA, Boyd GD, Mathews FS . Detailed active site configuration of a new crystal form of methanol dehydrogenase from Methylophilus W3A1 at 1.9 A resolution . Biochemistry . 38 . 4 . 1214–20 . January 1999 . 9930981 . 10.1021/bi9822574 .
8. Afolabi PR, Mohammed F, Amaratunga K, Majekodunmi O, Dales SL, Gill R, Thompson D, Cooper JB, Wood SP, Goodwin PM, Anthony C . Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L) . Biochemistry . 40 . 33 . 9799–809 . August 2001 . 11502173 . 10.1021/bi002932l .
9. Anthony C, Williams P . The structure and mechanism of methanol dehydrogenase . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics . 1647 . 1–2 . 18–23 . April 2003 . 12686102 . 10.1016/S1570-9639(03)00042-6 .
10. Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB . The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens . Acta Crystallographica Section D . 61 . Pt 1 . 75–9 . January 2005 . 15608378 . 10.1107/S0907444904026964 . free .