Mesobacillus Explained

Mesobacillus is a genus of gram-positive or gram-variable, rod-shaped bacteria in the family Bacillaceae within the order Bacillales.[1] [2] The type species for this genus is Mesobacillus jeotgali.[3]

Members of Mesobacillus was transferred from the genus Bacillus. Bacillus is a large, phylogenetically complicated genus, with unclear interspecies evolutionary relationships[4] due to the vague criteria used to assign species into the genus.[5] The efforts of multiple phylogenetic and comparative genomics analyses studies have transferred many Bacillus species into novel genera such as Virgibacillus, Solibacillus, Brevibacillus and Ectobacillus.[6] [7] [8] [9] The genus itself has also been emended and restricted to only include species closely related to Bacillus subtilis and Bacillus cereus.

The name Mesobacillus is derived from its taxonomic position in phylogenetic trees. The prefix "meso-" comes from the Greek adjective mesos, and translates to "middle". Bacillus comes from the Latin noun bacillus, referring to both 'a small staff or rod' and Bacillus, the bacterial genus. Put together, the name Mesobacillus refers to a genus in between other Bacillus.

Biochemical Characteristics and Molecular Signatures

Members of the genus Mesobacillus are either aerobic or facultatively anaerobic. They mostly demonstrate endospore formation under adverse environmental or nutritional conditions. Species can be found in a variety of environments, ranging from soil, human gut, groundwater, wastewater systems, hypersalinic lakes and thermal aquifers. Cells can be either motile or non-motile. Some specific species are high salt tolerant and even require high salt concentration for growth (ie. Mesobacillus persicus).[10] Mesobacillus can survive in temperatures ranging from 10°C to 45°C, but optimal growth occurs in the range of 25-37°C.

Genome sequence analyses have identified three conserved signature indels (CSIs) uniquely found in members of Mesobacillus in the proteins PhoH family protein, TrkA family potassium uptake protein and a hypothetical protein. These molecular markers provide a reliable means to distinguish Mesobacillus species from other Bacillaceae genera and bacteria.

Taxonomy

As of May 2021, there are a total of 14 species with validly published names in the genus Mesobacillus. In phylogenetic trees constructed based on concatenated sequences from various datasets of conserved proteins and 16S rRNA sequences, members of this genus group together and forms a monophyletic branch. This grouping is also reflected n the Genome Taxonomy Database (GTDB). [11]

The non-validly published species "Bacillus mediterraneensis" has been found to branch with other members of Mesobacillus as well as share molecular markers unique to the clade. However, transfer of these species into Mesobacillus was not proposed due to the lack of strain culture information. This indicates the requirement for additional analyses of this clade as more genomic and strain information become available.

Notes and References

  1. Patel. Sudip. Gupta. Radhey S.. 2020-01-01. A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov.. International Journal of Systematic and Evolutionary Microbiology. en. 70. 1. 406–438. 10.1099/ijsem.0.003775. 1466-5026. 31617837. free.
  2. Book: 2005. Brenner. Don J.. Krieg. Noel R.. Staley. James T.. Garrity. George M.. Boone. David R.. De Vos. Paul. Goodfellow. Michael. Rainey. Fred A.. Schleifer. Karl-Heinz. Bergey's Manual® of Systematic Bacteriology. 10.1007/0-387-28022-7. 978-0-387-24144-9.
  3. Web site: Genus: Mesobacillus. 2021-05-25. lpsn.dsmz.de. en.
  4. Ash. Carol. Farrow. J.A.E.. Wallbanks. Sally. Collins. M.D.. 2008-06-28. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Letters in Applied Microbiology. en. 13. 4. 202–206. 10.1111/j.1472-765X.1991.tb00608.x. 82988953.
  5. Logan. N. A.. Berge. O.. Bishop. A. H.. Busse. H.-J.. De Vos. P.. Fritze. D.. Heyndrickx. M.. Kampfer. P.. Rabinovitch. L.. Salkinoja-Salonen. M. S.. Seldin. L.. 2009-06-30. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. International Journal of Systematic and Evolutionary Microbiology. 59. 8. 2114–2121. 10.1099/ijs.0.013649-0. 19567583. 1466-5026. free.
  6. Heyndrickx. M.. Lebbe. L.. Kersters. K.. Hoste. B.. De Wachter. R.. De Vos. P.. Forsyth. G.. Logan. N. A.. 1999-07-01. Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. International Journal of Systematic and Evolutionary Microbiology. 49. 3. 1083–1090. 10.1099/00207713-49-3-1083. 1466-5026. 10425765. free.
  7. Shida. O.. Takagi. H.. Kadowaki. K.. Komagata. K.. 1996-10-01. Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.. International Journal of Systematic Bacteriology. en. 46. 4. 939–946. 10.1099/00207713-46-4-939. 0020-7713. 8863420. free.
  8. Mual. Poonam. Singh. Nitin Kumar. Verma. Ashish. Schumann. Peter. Krishnamurthi. Srinivasan. Dastager. Syed. Mayilraj. Shanmugam. 2016-05-01. Reclassification of Bacillus isronensis Shivaji et al. 2009 as Solibacillus isronensis comb. nov. and emended description of genus Solibacillus Krishnamurthi et al. 2009. International Journal of Systematic and Evolutionary Microbiology. en. 66. 5. 2113–2120. 10.1099/ijsem.0.000982. 1466-5026. 26907585. free.
  9. Gupta. Radhey S.. Patel. Sudip. Saini. Navneet. Chen. Shu. 2020-11-01. Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species. International Journal of Systematic and Evolutionary Microbiology. en. 70. 11. 5753–5798. 10.1099/ijsem.0.004475. 1466-5026. 33112222. free.
  10. Didari. Maryam. Amoozegar. Mohammad Ali. Bagheri. Maryam. Mehrshad. Maliheh. Schumann. Peter. Spröer. Cathrin. Sánchez-Porro. Cristina. Ventosa. Antonio. 2013-04-01. Bacillus persicus sp. nov., a halophilic bacterium from a hypersaline lake. International Journal of Systematic and Evolutionary Microbiology. 63. Pt_4. 1229–1234. 10.1099/ijs.0.042689-0. 22771682. 1466-5026.
  11. Web site: GTDB - Tree. 2021-05-25. gtdb.ecogenomic.org.

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