MeRIPseq explained

MeRIPseq^[1] (or MeRIP-seq) stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications, developed by Kate Meyer et al. while working in the laboratory of Sammie Jaffrey at Cornell University Graduate School of Medical Sciences. It is also called m6A-seq.^[2]

A variation of the MerIP-seq method was coined by Benjamin Delatte and colleagues in 2016. This variant, called hMerIP-seq (hydroxymethylcytosine RNA immunoprecipitation), uses an antibody that specifically recognizes 5-hydroxymethylcytosine, a modified RNA base affecting in vitro translation and brain development in Drosophila.^[3]

Notes and References

1. Meyer. Kate D.. Saletore, Yogesh . Zumbo, Paul . Elemento, Olivier . Mason, Christopher E. . Jaffrey, Samie R. . Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons. Cell. 31 May 2012. 149. 7. 1635–1646. 10.1016/j.cell.2012.05.003. 22608085. 3383396.
2. Dominissini. Dan . Moshitch-Moshkovitz, Sharon . Schwartz, Schraga . Salmon-Divon, Mali . Ungar, Lior . Osenberg, Sivan . Cesarkas, Karen . Jacob-Hirsch, Jasmine . Amariglio, Ninette . Kupiec, Martin . Sorek, Rotem . Rechavi, Gideon . 3517716 . Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature. 28 April 2012. 485. 7397. 201–206. 10.1038/nature11112. 22575960. 2012Natur.485..201D .
3. Delatte. Benjamin. Transcriptome-wide distribution and function of RNA hydroxymethylcytosine. Science. 2016. 351. 6270. 282–285. 26816380. 10.1126/science.aac5253. 2016Sci...351..282D. free.