| Mayo River | |
| Name Other: | Tributary to Dan River |
| Map Size: | 300 |
| Pushpin Map: | USA North Carolina#USA |
| Pushpin Map Size: | 300 |
| Pushpin Map Caption: | Location of Mayo River mouth |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | North Carolina |
| Subdivision Type3: | County |
| Subdivision Name3: | Rockingham |
| Subdivision Type4: | City |
| Subdivision Name4: | Mayodan |
| Length: | 15.58miles[1] |
| Discharge1 Location: | Mayodan River |
| Discharge1 Avg: | 416.64cuft/s at mouth with Dan River[2] |
| Source1: | confluence of North and South Mayo Rivers |
| Source1 Location: | about 6 miles northeast of Ayersville, North Carolina |
| Source1 Coordinates: | 36.5403°N -78.9892°W[3] |
| Source1 Elevation: | 698feet[4] |
| Mouth: | Dan River |
| Mouth Location: | Madison, North Carolina |
| Mouth Coordinates: | 36.39°N -78.9519°W |
| Mouth Elevation: | 538feet |
| Progression: | south-southeast |
| River System: | Dan River |
| Tributaries Left: | Buffalo Creek Hickory Creek |
| Tributaries Right: | Pawpaw Creek |
| Bridges: | Anglin Mill Road, T. Clarence Stone Highway, US 220, JJ Webster Highway, Dan Valley Road |
The Mayo River is a tributary of the Dan River, which in turn is a tributary of the Roanoke River. All three rivers flow through the U.S. states of Virginia and North Carolina. It is named for Major William Mayo (ca. 1685-1744).
The Mayo River is formed by two main branches, the North Fork Mayo River and the South Fork Mayo River. Both forks rise in Patrick County, Virginia, gathering the waters of many tributary streams. The two forks join in northwestern Rockingham County, North Carolina, forming the Mayo River proper, which then flows south into joining the Dan River near the towns of Mayodan, North Carolina and Madison, North Carolina.