| Official Name: | Matyushkino |
| Native Name: | Матюшкино |
| Settlement Type: | Village |
| Pushpin Map: | Russia Vologda Oblast#Russia |
| Subdivision Type: | Country |
| Subdivision Name: | Russia |
| Subdivision Name1: | Vologda Oblast |
| Subdivision Name3: | Mezhdurechensky District |
| Subdivision Type4: | Municipality |
| Utc Offset1: | +3:00 |
| Coordinates: | 59.2°N 83°W[1] |
Matyushkino (Russian: Матюшкино) is a rural locality (a village) in Staroselskoye Rural Settlement, Mezhdurechensky District, Vologda Oblast, Russia. The population was 3 as of 2002.[2]
Matyushkino is located 26 km southwest of Shuyskoye (the district's administrative centre) by road. Svyatogorye is the nearest rural locality.[3]