| Matupiri River | |
| Pushpin Map: | Brazil |
| Source1 Location: | Amazonas state |
| Source1 Coordinates: | -5.299°N -61.8527°W |
| Mouth Coordinates: | -4.5396°N -60.4697°W |
| Subdivision Type1: | Country |
| Subdivision Name1: | Brazil |
The Matupiri River (Portuguese: Rio Matupiri) is a river of Amazonas state in north-western Brazil.It is a tributary of the Igapó-Açu River.
The Matupiri River forms in the Rio Amapá Sustainable Development Reserve where the Jutaí and Novo rivers converge.The Matupiri River runs through the centre of the Matupiri State Park from southwest to northeast, and provides the main way to access the interior of the park. It then runs through the northwest corner of the Matupiri Sustainable Development Reserve and then through the Cunhã-Sapucaia Indigenous Territory, within which it enters the Igapó-Açu River.