This is the progression of world record improvements of the 400 metres W65 division of Masters athletics.
| Hand | Auto | Athlete | Nationality | Birthdate | Age | Location | Date | Ref | |
|---|---|---|---|---|---|---|---|---|---|
| 1:07.23 | Elizabeth Deak | 24 February 1958 | Greensboro | 21 July 2023 | [1] | ||||
| 1:08.08 | Karla Del Grande | 27 March 1953 | Toronto | 12 July 2019 | |||||
| 1:08.21 | Diane Palmason | 15 March 1938 | Eugene | 8 August 2003 | |||||
| 1:11.45 | Carolyn Sue Cappetta | 1936 | 65 | Brisbane | 13 July 2001 | ||||
| 1:13.71 | Anna Mangler | 14 January 1923 | Eugene | 5 August 1989 | |||||
| 1:14.31 | Paula Schneiderhan | 16 November 1921 | Melbourne | 5 December 1987 | |||||
| 1:16.7 | Winifred Audrey Reid | 1915 | 68 | Germiston | 19 May 1984 |