This is the progression of world record improvements of the 400 metres W40 division of Masters athletics.
| Hand | Auto | Athlete | Nationality | Birthdate | Location | Date | |
|---|---|---|---|---|---|---|---|
| 52.50 | Geisa Aparecida Coutinho | 1 June 1980 | Bragança Paulista | 9 April 2021 | |||
| 53.05 | Maria Magnolia Figueiredo | 11 November 1963 | Bogotà | 11 July 2004 | |||
| 53.68 | Sara Montecinos | 8 March 1954 | Cali | 20 March 1994 | |||
| 55.38 | Maeve Kyle | 6 October 1928 | Edinburgh | 22 July 1970 |