This is the progression of world record improvements of the 400 metres M65 division of Masters athletics.
| Hand | Auto | Athlete | Nationality | Birthdate | Age | Location | Date | |
|---|---|---|---|---|---|---|---|---|
| 56.28 | Charles Allie | 20 August 1947 | Falls Church | 1 September 2012 | ||||
| 56.37 | Guido Müller | 22 December 1938 | Hof | 13 June 2004 | ||||
| 57.27 | Ralph Romain | 20 July 1932 | Durban | 25 July 1997 | ||||
| 57.52 | Berthold Neumann | 19 December 1930 | Malmö | 22 July 1996 | ||||
| 57.97 | Earl Fee | 22 March 1929 | Buffalo | 22 July 1995 | ||||
| 58.52 | Jim Law | 1926 | 65 | Syracuse | 27 June 1991 | |||
| 1:01.15 | Arne Pettersson | 24 March 1923 | Gothenburg | 3 September 1988 |