Master mix (PCR) explained
A master mix is a mixture containing precursors and enzymes used as an ingredient in polymerase chain reaction techniques in molecular biology. Such mixtures contain a mixture dNTPs (required as a substrate for the building of new DNA strands), MgCl2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer and come mixed in nuclease-free water.[1] [2] [3] [4]
Master mixes for real-time PCR include a fluorescent compound (frequently SYBR green), and the choice of mix also influence test sensitivity and consistency.[5]
Differences in the choice of master mixes can sometimes explain difference in experimental results, a particular case being the measurement of telomere length.[6] [7]
Notes and References
- Web site: PCR Master Mix . Sigma-Aldrich . Merck . en.
- Web site: GoTaq® G2 Master Mixes PCR Master Mix . www.promega.com.
- Web site: PCR Master Mix Bio-Rad . www.bio-rad.com . Bio Rad.
- Web site: PCR Master Mixes Chai . www.chaibio.com . Chai.
- Yang . Jianxin . Kemps-Mols . Berit . Spruyt-Gerritse . Marijke . Anholts . Jacqueline . Claas . Frans . Eikmans . Michael . The source of SYBR green master mix determines outcome of nucleic acid amplification reactions . BMC Research Notes . 4 June 2016 . 9 . 1 . 10.1186/s13104-016-2093-4. 27259280 . free . 4893258 .
- Jiménez . Karen M. . Forero . Diego A. . Effect of master mixes on the measurement of telomere length by qPCR . Molecular Biology Reports . 5 April 2018 . 45 . 4 . 633–638 . 10.1007/s11033-018-4175-y. 254833450 .
- Lin . Jue . Smith . Dana L. . Esteves . Kyle . Drury . Stacy . Telomere length measurement by qPCR – Summary of critical factors and recommendations for assay design . Psychoneuroendocrinology . January 2019 . 99 . 271–278 . 10.1016/j.psyneuen.2018.10.005. 30343983 . 6363640 .
