| Marlboro, Virginia | |
| Settlement Type: | Unincorporated community |
| Pushpin Map: | Virginia#USA |
| Pushpin Label: | Marlboro |
| Pushpin Label Position: | bottom |
| Subdivision Type: | Country |
| Subdivision Name: | United States |
| Subdivision Type1: | State |
| Subdivision Name1: | Virginia |
| Subdivision Type2: | County |
| Subdivision Name2: | Frederick |
| Unit Pref: | Imperial |
| Population As Of: | 2000 |
| Population Density Km2: | auto |
| Timezone: | Eastern (EST) |
| Utc Offset: | -5 |
| Timezone Dst: | EDT |
| Utc Offset Dst: | -4 |
| Coordinates: | 39.0814°N -78.3222°W |
| Postal Code Type: | ZIP codes |
| Blank Name: | FIPS code |
| Blank1 Name: | GNIS feature ID |
| Blank1 Info: | 1495901 |
Marlboro is an unincorporated community in Frederick County, Virginia, United States. It is located at the intersection of Middle Road (VA 628) and Cedar Creek Grade (VA 622) on Cedar Creek. Marlboro was originally known as Marlborough and Marlboroughtown.