| Mario Holek | |
| Fullname: | Mario Holek |
| Birth Date: | 1986 10, df=yes |
| Birth Place: | Brno, Czechoslovakia |
| Height: | 1.85 m[1] |
| Position: | Centre back |
| Youthyears1: | 1990–2004 |
| Youthclubs1: | 1. FC Brno |
| Years1: | 2005–2007 |
| Clubs1: | 1. FC Brno |
| Caps1: | 67 |
| Goals1: | 4 |
| Years2: | 2008–2011 |
| Caps2: | 60 |
| Goals2: | 1 |
| Years3: | 2012–2017 |
| Caps3: | 124 |
| Goals3: | 2 |
| Years4: | 2017–2018 |
| Clubs4: | → Dukla Prague (loan) |
| Caps4: | 4 |
| Goals4: | 0 |
| Years5: | 2018 |
| Clubs5: | Příbram |
| Caps5: | 0 |
| Goals5: | 0 |
| Nationalyears1: | 2003 |
| Nationalcaps1: | 2 |
| Nationalgoals1: | 0 |
| Nationalyears2: | 2003 |
| Nationalcaps2: | 8 |
| Nationalgoals2: | 0 |
| Nationalyears3: | 2004–2005 |
| Nationalcaps3: | 10 |
| Nationalgoals3: | 0 |
| Nationalyears4: | 2006–2008 |
| Nationalcaps4: | 19 |
| Nationalgoals4: | 1 |
| Nationalyears5: | 2009–2015 |
| Nationalteam5: | Czech Republic |
| Nationalcaps5: | 8 |
| Nationalgoals5: | 0 |
| Club-Update: | 23 December 2018 |
Mario Holek (born 28 October 1986) is a retired Czech footballer who represented the Czech Republic national football team.
He started his career in his hometown of Brno in the Czech Republic playing for the local club 1. FC Brno, where he spent five seasons. In January 2008, Holek was transferred to Ukraine, to a Premier Division club Dnipro Dnipropetrovsk. In December 2011 he joined Sparta as a free agent. Later in his career he played for Dukla Prague and Příbram.