| Margaret V. Gillespie | |
| State House: | South Dakota |
| State: | South Dakota |
| District: | 16th |
| Term Start: | 2001 |
| Succeeded: | Incumbent |
| Birth Date: | 15 February 1969 |
| Birth Place: | Hawarden, Iowa, U.S. |
| Profession: | Attorney |
| Party: | Democratic |
Margaret Vandemore Gillespie (born February 15, 1969) is a Democratic member of the South Dakota House of Representatives, representing the 16th District since 2001. Her district includes Lincoln and Union counties.
Gillespie is an attorney living in Hudson, South Dakota.