| Season: | 2000–01 |
| Dates: | 19 August 2000 – 30 April 2001 |
| Competition: | Maltese Premier League |
| Winners: | Valletta (18th title) |
| Relegated: | Rabat Ajax Xgħajra Tornadoes |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Valletta |
| Continentalcup2: | UEFA Cup |
| Continentalcup2 Qualifiers: | Sliema Wanderers Birkirkara |
| Continentalcup3: | UEFA Intertoto Cup |
| Continentalcup3 Qualifiers: | Hibernians |
| League Topscorer: | Michael Mifsud (30 goals) |
| Highest Scoring: | Xgħajra Tornadoes 0–11 Sliema Wanderers |
| Matches: | 132 |
| Total Goals: | 506 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Maltese Premier League (known as the Rothmans Premier League for sponsorship reasons) was the 21st season of the Maltese Premier League, and the 86th season of top-tier football in Malta. The league started on 19 August 2000 with Birkirkara as the defending champions after their first title in the previous season.
The following teams were promoted from the First Division at the start of the season:
From the previous Premier League season, the following teams were relegated to the First Division:
The teams placed in the first six positions in the league table qualified for the Top Six, and the points obtained during the first phase were halved (and rounded up) before the start of second phase. As a result, the teams started with the following points before the second phase: Valletta 21 points, Sliema Wanderers 20, Birkirkara 19, Hibernians 18, Floriana 17 and Ħamrun Spartans 13.
The teams which finished in the last four league positions were placed in the play-out and at the end of the phase the two lowest-placed teams were relegated to the First Division. The points obtained during the first phase were halved (and rounded up) before the start of second phase. As a result, the teams started with the following points before the second phase: Pietà Hotspurs 9 points, Naxxar Lions 6, Rabat Ajax 5, Xgħajra Tornadoes 1.
| Rank | Player | Club | Goals[1] |
|---|---|---|---|
| 1 |  Michael Mifsud | Sliema Wanderers | 30 |
| 2 | Adrian Mifsud | Hibernians | 23 |
| 3 |  Chris Oretan | Valletta | 22 |
| Orosco Anonam | Sliema Wanderers | ||
| 5 | Michael Galea | Birkirkara | 21 |
| 6 | Malcolm Licari | Pietà Hotspurs | 19 |
| 7 | Gilbert Agius | Valletta | 15 |
| 8 |  Rufin Oba | Floriana | 12 |
| 9 |  Eduardo do Nascimento | Floriana | 11 |
| Massimo Grima | Sliema Wanderers | ||
| Ivan Woods | Pietà Hotspurs |
| Player[2] | For | Against | Result | Date | |
|---|---|---|---|---|---|
| Paul Zammit | Ħamrun Spartans | Xgħajra Tornadoes | 3–0 | ||
| Michael Mifsud | Sliema Wanderers | Rabat Ajax | 6–0 | ||
| Michael Mifsud6 | Sliema Wanderers | Xgħajra Tornadoes | 11–0 | ||
| Orosco Anonam | Sliema Wanderers | Xgħajra Tornadoes | 11–0 | ||
| Ndubisi Chukunyere | Hibernians | Floriana | 4–1 | ||
| Stefan Sultana4 | Hibernians | Xgħajra Tornadoes | 6–0 | ||
| Rufin Oba | Floriana | Rabat Ajax | 7–0 | ||
| Michael Mifsud | Sliema Wanderers | Ħamrun Spartans | 6–1 | ||
| Malcolm Licari | Pietà Hotspurs | Naxxar Lions | 4–0 | ||
| Michael Mifsud | Sliema Wanderers | Naxxar Lions | 7–0 |