| Mailley-et-Chazelot | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason ville fr Mailley-et-Chazelot 70.svg |
| Coordinates: | 47.5369°N 6.0525°W |
| Insee: | 70324 |
| Postal Code: | 70000 |
| Arrondissement: | Vesoul |
| Canton: | Scey-sur-Saône-et-Saint-Albin |
| Mayor: | Bertrand Rezard[1] |
| Term: | 2020 - 2026 |
| Elevation Min M: | 249 |
| Elevation Max M: | 463 |
| Area Km2: | 25.03 |
Mailley-et-Chazelot (in French pronounced as /majɛ e ʃazlo/) is a commune in the Haute-Saône department in the region of Bourgogne-Franche-Comté in eastern France.