| Magdalena Sibylle of Holstein-Gottorp | |
| House: | House of Oldenburg |
| Father: | Frederick III, Duke of Holstein-Gottorp |
| Mother: | Duchess Marie Elisabeth of Saxony |
| Spouse: | Gustav Adolph, Duke of Mecklenburg-Güstrow |
| Issue: | Christine of Mecklenburg-Güstrow Charles of Mecklenburg-Güstrow Hedwig of Mecklenburg-Güstrow Louise of Mecklenburg-Güstrow Elisabeth of Mecklenburg-Güstrow |
| Birth Date: | 24 November 1631 |
| Birth Place: | Gottorp Castle |
| Death Date: | 22 September 1719 (aged 87) |
| Death Place: | Güstrow |
Magdalena Sibylle of Holstein-Gottorp (also spelled Magdalena Sibylla; 24 November 1631 at Gottorp Castle - 22 September 1719 in Güstrow) was a Duchess of Holstein-Gottorp by birth and by marriage Duchess of Mecklenburg-Güstrow. From 1654 to 1695, she was the consort of Duke Gustav Adolph of Mecklenburg-Güstrow.
Magdalene Sybille was the daughter of Duke Frederick III of Schleswig-Holstein-Gottorp (1597–1659) and his wife Marie Elisabeth of Saxony (1610–1684).
After her husband's death, she remained a widow for 26 years. She retained a small court in Güstrow. However, after the Mecklenburg-Güstrow line of Dukes had died out, the court in Güstrow lost its former glory and significance.
On 28 December 1654, she married Gustav Adolph, the ruling Duke of Mecklenburg-Güstrow. They had eleven children but no surviving male heirs. This leads to a succession dispute that was settled by the Partition of Hamburg (1701), which redivided the interior of the Duchy of Mecklenburg among the surviving lines.