MYOT explained

Myotilin is a protein that in humans is encoded by the MYOT gene.[1] [2] [3] Myotilin (myofibrillar titin-like protein) also known as TTID (TiTin Immunoglobulin Domain) is a muscle protein that is found within the Z-disc of sarcomeres.

Structure

Myotilin is a 55.3 kDa protein composed of 496 amino acids.[4] Myotilin was originally identified as a novel alpha-actinin binding partner with two Ig-like domains, that localized to the Z-disc.[5] The I-type Ig-like domains reside at the C-terminal half, and are most homologous to Ig domains 2-3 of palladin and Ig domains 4-5 of myopalladin and more distantly related to Z-disc Ig domains 7 and 8 of titin. The C-terminal region hosts the binding sites for Z-band proteins, and 2 Ig domains are the site of homodimerization for myotilin.[6] By contrast, the N-terminal part of myotilin is unique, consisting of a serine-rich region with no homology to known proteins. Several disease-associated mutations involve serine residues within the serine-rich domain.[7] Myotilin expression in human tissues is mainly restricted to striated muscles and nerves. In muscles, myotilin is predominantly found within the Z-discs. Myotilin forms homodimers and binds alpha-actinin, actin,[8] Filamin C,[9] FATZ-1, FATZ-2[10] and ZASP.[11]

Function

Myotilin is a structural protein that, along with titin and alpha-actinin give structural integrity to sarcomeres at Z-discs in striated muscle. Myotilin induces the formation of actin bundles in vitro and in non-muscle cells. A ternary complex myotilin/actin/alpha-actinin can be observed in vitro and actin bundles formed under these conditions appear more tightly packed than those induced by alpha-actinin alone. It was demonstrated that myotilin stabilizes F-actin by slowing down the disassembly rate. Ectopic overexpression of truncated myotilin causes the disruption of nascent myofibrils and the co-accumulation of myotilin and titin in amorphous cytoplasmic precipitates. In mature sarcomeres, wild-type myotilin colocalizes with alpha-actinin and Z-disc titin, showing the striated pattern typical of sarcomeric proteins. Targeted disruption of the myotilin gene in mice does not cause significant alterations in muscle function.[12] On the other hand, transgenic mice with mutated myotilin develop muscle dystrophy.[13]

Clinical significance

Myotilin is mutated in various forms of muscular dystrophy: Limb-Girdle Muscular Dystrophy type 1A (LGMD1A), Myofibrillar Myopathy (MFM), Spheroid Body Myopathy and Distal Myopath.[7] The mechanism underlying the pathology is still under investigation. It has been shown that actin binding properties of myotilin housing pathogenic mutations (Ser55Phe, Thr57Ile, Ser60Cys, and Ser95Ile) are normal,[14] albeit with a slower rate of degradation.[15] Surprisingly, YFP-fusion constructs of myotilin mutants (Ser55Phe, Ser55Ile, Thr57Ile, Ser60Cys, Ser60Phe, Ser95Ile, Arg405Lys) localized normally to Z-discs and exhibited normal dynamics in muscle cells.[16]

Further reading

External links

Notes and References

  1. Godley LA, Lai F, Liu J, Zhao N, Le Beau MM . TTID: A novel gene at 5q31 encoding a protein with titin-like features . Genomics . 60 . 2 . 226–33 . Nov 1999 . 10486214 . 10.1006/geno.1999.5912 .
  2. Salmikangas P, Mykkanen OM, Gronholm M, Heiska L, Kere J, Carpen O . Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy . Hum Mol Genet . 8 . 7 . 1329–36 . Aug 1999 . 10369880 . 10.1093/hmg/8.7.1329 . 16176213 . free .
  3. Web site: Entrez Gene: MYOT myotilin.
  4. Web site: Myotilin protein information . Cardiac Organellar Protein Atlas Knowledgebase (COPaKB) . 2015-03-23 . 2015-09-24 . https://web.archive.org/web/20150924025611/http://www.heartproteome.org/copa/ProteinInfo.aspx?QType=Protein%20ID&QValue=Q9JIF9 . dead .
  5. Salmikangas P, Mykkänen OM, Grönholm M, Heiska L, Kere J, Carpén O . Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy . Human Molecular Genetics . 8 . 7 . Jul 1999 . 10369880 . 10.1093/hmg/8.7.1329 . 1329–36. 16176213 . free .
  6. Shalaby S, Mitsuhashi H, Matsuda C, Minami N, Noguchi S, Nonaka I, Nishino I, Hayashi YK . Defective myotilin homodimerization caused by a novel mutation in MYOT exon 9 in the first Japanese limb girdle muscular dystrophy 1A patient . Journal of Neuropathology and Experimental Neurology . 68 . 6 . Jun 2009 . 19458539 . 10.1097/NEN.0b013e3181a7f703 . 701–7. free .
  7. Selcen D . Myofibrillar myopathies . Neuromuscular Disorders . 21 . 3 . Mar 2011 . 21256014 . 10.1016/j.nmd.2010.12.007 . 161–71 . 3052736.
  8. Salmikangas P, van der Ven PF, Lalowski M, Taivainen A, Zhao F, Suila H, Schröder R, Lappalainen P, Fürst DO, Carpén O . Myotilin, the limb-girdle muscular dystrophy 1A (LGMD1A) protein, cross-links actin filaments and controls sarcomere assembly . Human Molecular Genetics . 12 . 2 . Jan 2003 . 12499399 . 10.1093/hmg/ddg020 . 189–203. free .
  9. van der Ven PF, Wiesner S, Salmikangas P, Auerbach D, Himmel M, Kempa S, Hayess K, Pacholsky D, Taivainen A, Schröder R, Carpén O, Fürst DO . Indications for a novel muscular dystrophy pathway. gamma-filamin, the muscle-specific filamin isoform, interacts with myotilin . The Journal of Cell Biology . 151 . 2 . Oct 2000 . 11038172 . 10.1083/jcb.151.2.235 . 2192634 . 235–248.
  10. Gontier Y, Taivainen A, Fontao L, Sonnenberg A, van der Flier A, Carpen O, Faulkner G, Borradori L . The Z-disc proteins myotilin and FATZ-1 interact with each other and are connected to the sarcolemma via muscle-specific filamins . Journal of Cell Science . 118 . Pt 16 . Aug 2005 . 16076904 . 10.1242/jcs.02484 . 3739–49. free .
  11. von Nandelstadh P, Ismail M, Gardin C, Suila H, Zara I, Belgrano A, Valle G, Carpen O, Faulkner G . A class III PDZ binding motif in the myotilin and FATZ families binds enigma family proteins: a common link for Z-disc myopathies . Molecular and Cellular Biology . 29 . 3 . Feb 2009 . 19047374 . 10.1128/MCB.01454-08 . 822–34 . 2630697.
  12. Moza M et al . 2007 . Targeted deletion of the muscular dystrophy gene myotilin does not perturb muscle structure or function in mice . Mol Cell Biol . 27 . 1. 244–252 . 10.1128/mcb.00561-06. 17074808 . 1800670.
  13. Garvey SM et al . 2006 . Transgenic mice expressing the myotilin T57I mutation unite the pathology associated with LGMD1A and MFM . Hum Mol Genet . 15 . 15. 2348–62 . 10.1093/hmg/ddl160 . 16801328. free .
  14. von Nandelstadh P, Grönholm M, Moza M, Lamberg A, Savilahti H, Carpén O . Actin-organising properties of the muscular dystrophy protein myotilin . Experimental Cell Research . 310 . 1 . Oct 2005 . 16122733 . 10.1016/j.yexcr.2005.06.027 . 131–9.
  15. von Nandelstadh P, Soliymani R, Baumann M, Carpen O . Analysis of myotilin turnover provides mechanistic insight into the role of myotilinopathy-causing mutations . The Biochemical Journal . 436 . 1 . May 2011 . 21361873 . 10.1042/BJ20101672 . 113–21.
  16. Wang J, Dube DK, Mittal B, Sanger JM, Sanger JW . Myotilin dynamics in cardiac and skeletal muscle cells . Cytoskeleton . 68 . 12 . Dec 2011 . 22021208 . 10.1002/cm.20542 . 661–70 . 3240742.