MTIF2 explained

Translation initiation factor IF-2, mitochondrial is a protein that in humans is encoded by the MTIF2 gene.^{[1] [2] [3]}

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene.^[3]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Overman RG, Enderle PJ, Farrow JM, etal . The human mitochondrial translation initiation factor 2 gene (MTIF2): transcriptional analysis and identification of a pseudogene . Biochim. Biophys. Acta . 1628 . 3 . 195–205 . 2003 . 12932832 . 10.1016/s0167-4781(03)00144-1.
• Bouwmeester T, Bauch A, Ruffner H, etal . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086 . 11683986 .
• Miura E, Kato Y, Matsushima R, etal . The Balance between Protein Synthesis and Degradation in Chloroplasts Determines Leaf Variegation in Arabidopsis yellow variegated Mutants . Plant Cell . 19 . 4 . 1313–28 . 2007 . 17416734 . 10.1105/tpc.106.049270 . 1913758 .

Notes and References

1. Bonner DS, Wiley JE, Farwell MA . Assignment1 of the mitochondrial translational initiation factor 2 gene (MTIF2) to human chromosome 2 bands p16→p14 by in situ hybridization and with somatic cell hybrids . Cytogenet Cell Genet . 83 . 1–2 . 80–1 . Mar 1999 . 9925935 . 10.1159/000015133 . 23290955 .
2. Ma L, Spremulli LL . Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA . J Biol Chem . 270 . 4 . 1859–65 . Feb 1995 . 7829522 . 10.1074/jbc.270.4.1859 . free .
3. Web site: Entrez Gene: MTIF2 mitochondrial translational initiation factor 2.