MRPS30 explained

28S ribosomal protein S30, mitochondrial is a protein that in humans is encoded by the MRPS30 gene.^{[1] [2] [3]}

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.^[3]

Further reading

• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Zhang Z, Gerstein M . Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome . Genomics . 81 . 5 . 468–80 . 2003 . 12706105 . 10.1016/S0888-7543(03)00004-1 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Kenmochi N, Suzuki T, Uechi T, etal . The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders . Genomics . 77 . 1–2 . 65–70 . 2001 . 11543634 . 10.1006/geno.2001.6622 .
• Wiemann S, Weil B, Wellenreuther R, etal . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 .
• Zhang QH, Ye M, Wu XY, etal . Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells . Genome Res. . 10 . 10 . 1546–60 . 2001 . 11042152 . 10.1101/gr.140200 . 310934 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .

Notes and References

1. Carim L, Sumoy L, Nadal M, Estivill X, Escarceller M . Cloning, expression, and mapping of PDCD9, the human homolog of Gallus gallus pro-apoptotic protein p52 . Cytogenet Cell Genet . 87 . 1–2 . 85–8 . May 2000 . 10640817 . 10.1159/000015397 . 28253818 .
2. Cavdar Koc E, Burkhart W, Blackburn K, Moseley A, Spremulli LL . The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present . J Biol Chem . 276 . 22 . 19363–74 . May 2001 . 11279123 . 10.1074/jbc.M100727200 . free . 10.1.1.501.8919 .
3. Web site: Entrez Gene: MRPS30 mitochondrial ribosomal protein S30.