Mitochondrial ribosomal protein L32 explained

39S ribosomal protein L32, mitochondrial is a protein that in humans is encoded by the MRPL32 gene.^{[1] [2]}

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.^[3]

Further reading

• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Suzuki T, Terasaki M, Takemoto-Hori C, etal . Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria . J. Biol. Chem. . 276 . 24 . 21724–36 . 2001 . 11279069 . 10.1074/jbc.M100432200 . free .
• Koc EC, Burkhart W, Blackburn K, etal . The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present . J. Biol. Chem. . 276 . 47 . 43958–69 . 2001 . 11551941 . 10.1074/jbc.M106510200 . free .
• Bommel H, Xie G, Rossoll W, etal . Missense mutation in the tubulin-specific chaperone E (Tbce) gene in the mouse mutant progressive motor neuronopathy, a model of human motoneuron disease . J. Cell Biol. . 159 . 4 . 563–9 . 2003 . 12446740 . 10.1083/jcb.200208001 . 2173089 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Scherer SW, Cheung J, MacDonald JR, etal . Human chromosome 7: DNA sequence and biology . Science . 300 . 5620 . 767–72 . 2003 . 12690205 . 2882961 . 10.1126/science.1083423 . 2003Sci...300..767S .
• Zhang Z, Gerstein M . Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome . Genomics . 81 . 5 . 468–80 . 2003 . 12706105 . 10.1016/S0888-7543(03)00004-1 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Oh JH, Yang JO, Hahn Y, etal . Transcriptome analysis of human gastric cancer . Mamm. Genome . 16 . 12 . 942–54 . 2006 . 16341674 . 10.1007/s00335-005-0075-2 . 69278 .

Notes and References

1. Kenmochi N, Suzuki T, Uechi T, Magoori M, Kuniba M, Higa S, Watanabe K, Tanaka T . The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders . Genomics . 77 . 1–2 . 65–70 . Sep 2001 . 11543634 . 10.1006/geno.2001.6622 .
2. Web site: Entrez Gene: MRPL32 mitochondrial ribosomal protein L32.
3. Web site: Entrez Gene: MRPL32 mitochondrial ribosomal protein L32.