Multimerin 1 Explained

Multimerin 1, also known as elastin microfibril interfacer 4 (EMILIN-4), is a protein that, in humans, is encoded by the MMRN1 gene.^{[1] [2] [3]}

Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is composed of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec/Quebec Platelet Disorder) were found to have a deficiency of platelet multimerin.^[3]

Further reading

• Hayward CP . Multimerin: a bench-to-bedside chronology of a unique platelet and endothelial cell protein--from discovery to function to abnormalities in disease . Clinical and Investigative Medicine . 20 . 3 . 176–87 . 1997 . 9189649 .
• Hayward CP, Kelton JG . Multimerin . Curr. Opin. Hematol. . 2 . 5 . 339–44 . 1998 . 9372017 . 10.1097/00062752-199502050-00003 . 220574123 .
• Hayward CP . Multimerin is found in the alpha-granules of resting platelets and is synthesized by a megakaryocytic cell line . J. Clin. Invest. . 91 . 6 . 2630–9 . 1993 . 8514871 . 10.1172/JCI116502 . 443327 . Bainton DF . Smith JW . Horsewood . P . Stead . R H . Podor . T J . Warkentin . T E . Kelton . J G .
• Hayward CP . An autosomal dominant, qualitative platelet disorder associated with multimerin deficiency, abnormalities in platelet factor V, thrombospondin, von Willebrand factor, and fibrinogen and an epinephrine aggregation defect . Blood . 87 . 12 . 4967–78 . 1996 . 8652809 . 10.1182/blood.V87.12.4967.bloodjournal87124967. Rivard GE . Kane WH . Drouin . J . Zheng . S . Moore . JC . Kelton . JG . free .
• Hayward CP . Studies of multimerin in human endothelial cells . Blood . 91 . 4 . 1304–17 . 1998 . 9454761 . 10.1182/blood.V91.4.1304. Cramer EM . Song Z . Zheng . S . Fung . R . Massé . JM . Stead . RH . Podor . TJ . free .
• Maurer-Spurej E, Kahr WH, Carter CJ, Pittendreigh C, Cameron M, Cyr TD . The value of proteomics for the diagnosis of a platelet-related bleeding disorder . Platelets . 19 . 5 . 342–51 . 2008 . 18791940. 10.1080/09537100802010547 . 31920329 .
• Polgár J, Magnenat E, Wells TN, Clemetson KJ . Platelet glycoprotein Ia* is the processed form of multimerin--isolation and determination of N-terminal sequences of stored and released forms . Thromb. Haemost. . 80 . 4 . 645–8 . 1999 . 9798985 .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . Feingold EA . Grouse LH . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Gevaert K . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nat. Biotechnol. . 21 . 5 . 566–9 . 2004 . 12665801 . 10.1038/nbt810 . Goethals M . Martens L . Van Damme . Jozef . Staes . An . Thomas . Grégoire R. . Vandekerckhove . Joël . 23783563 .
• Jeimy SB . Identification of the MMRN1 binding region within the C2 domain of human factor V . J. Biol. Chem. . 279 . 49 . 51466–71 . 2005 . 15452129 . 10.1074/jbc.M409866200 . Woram RA . Fuller N . Quinn-Allen . MA . Nicolaes . GA . Dahlbäck . B . Kane . WH . Hayward . CP . free .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . Wagner L . Feingold EA . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Hayward CP . Human platelets contain forms of factor V in disulfide-linkage with multimerin . Thromb. Haemost. . 92 . 6 . 1349–57 . 2005 . 15583744 . 10.1160/TH03-02-0123 . Fuller N . Zheng S . Adam . F . Jeimy . SB . Horsewood . I . Quinn-Allen . MA . Kane . WH . 3935661 .
• Lewandrowski U, Moebius J, Walter U, Sickmann A . Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach . Mol. Cell. Proteomics . 5 . 2 . 226–33 . 2006 . 16263699 . 10.1074/mcp.M500324-MCP200 . free .
• Liu T . Human Plasma N-Glycoproteome Analysis by Immunoaffinity Subtraction, Hydrazide Chemistry, and Mass Spectrometry . J. Proteome Res. . 4 . 6 . 2070–80 . 2006 . 16335952 . 10.1021/pr0502065 . 1850943 . Qian WJ . Gritsenko MA . Camp . David G. . Monroe . Matthew E. . Moore . Ronald J. . Smith . Richard D. .
• Adam F . Analyses of cellular multimerin 1 receptors: in vitro evidence of binding mediated by alphaIIbbeta3 and alphavbeta3 . Thromb. Haemost. . 94 . 5 . 1004–11 . 2006 . 16363244 . 10.1160/TH05-02-0140 . Zheng S . Joshi N . Kelton . David S. . Sandhu . Amin . Suehiro . Youko . Jeimy . Samira B. . Santos . Aurelio V. . Massé . Jean-Marc . 84366459 .

Notes and References

1. Hayward CP, Hassell JA, Denomme GA, Rachubinski RA, Brown C, Kelton JG . The cDNA sequence of human endothelial cell multimerin. A unique protein with RGDS, coiled-coil, and epidermal growth factor-like domains and a carboxyl terminus similar to the globular domain of complement C1q and collagens type VIII and X . J Biol Chem . 270 . 31 . 18246–51 . Sep 1995 . 7629143 . 10.1074/jbc.270.31.18246. free .
2. Torres MD, Van Tuinen P, Kroner PA . The human multimerin gene MMRN maps to chromosome 4q22 . Cytogenet Cell Genet . 88 . 3–4 . 275–7 . Jun 2000 . 10828608 . 10.1159/000015537 . 39874017 .
3. Web site: Entrez Gene: MMRN1 multimerin 1.