MGAT3 explained

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the MGAT3 gene.^{[1] [2]}

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Further reading

• Kim YJ, Park JH, Kim KS, etal . Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III. . Gene . 170 . 2 . 281–3 . 1996 . 8666260 . 10.1016/0378-1119(95)00818-7 .
• Koyama N, Miyoshi E, Ihara Y, etal . Human N-acetylglucosaminyltransferase III gene is transcribed from multiple promoters. . Eur. J. Biochem. . 238 . 3 . 853–61 . 1996 . 8706690 . 10.1111/j.1432-1033.1996.0853w.x . free .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Dunham I, Shimizu N, Roe BA, etal . The DNA sequence of human chromosome 22. . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
• Ikeda Y, Koyota S, Ihara H, etal . Kinetic basis for the donor nucleotide-sugar specificity of beta1, 4-N-acetylglucosaminyltransferase III. . J. Biochem. . 128 . 4 . 609–19 . 2000 . 11011143 . 10.1093/oxfordjournals.jbchem.a022793. free .
• Song EY, Kang SK, Lee YC, etal . Expression of bisecting N-acetylglucosaminyltransferase-III in human hepatocarcinoma tissues, fetal liver tissues, and hepatoma cell lines of Hep3B and HepG2. . Cancer Invest. . 19 . 8 . 799–807 . 2002 . 11768033 . 10.1081/CNV-100107741 . 22792562 .
• Shibukawa Y, Takahashi M, Laffont I, etal . Down-regulation of hydrogen peroxide-induced PKC delta activation in N-acetylglucosaminyltransferase III-transfected HeLaS3 cells. . J. Biol. Chem. . 278 . 5 . 3197–203 . 2003 . 12427758 . 10.1074/jbc.M207870200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Sasai K, Ikeda Y, Ihara H, etal . Caveolin-1 regulates the functional localization of N-acetylglucosaminyltransferase III within the golgi apparatus. . J. Biol. Chem. . 278 . 28 . 25295–301 . 2003 . 12716887 . 10.1074/jbc.M301913200 . free .
• Millar JK, Christie S, Porteous DJ . Yeast two-hybrid screens implicate DISC1 in brain development and function. . Biochem. Biophys. Res. Commun. . 311 . 4 . 1019–25 . 2004 . 14623284 . 10.1016/j.bbrc.2003.10.101 .
• Isaji T, Gu J, Nishiuchi R, etal . Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration. . J. Biol. Chem. . 279 . 19 . 19747–54 . 2004 . 14998999 . 10.1074/jbc.M311627200 . free .
• Collins JE, Wright CL, Edwards CA, etal . A genome annotation-driven approach to cloning the human ORFeome. . Genome Biol. . 5 . 10 . R84 . 2005 . 15461802 . 10.1186/gb-2004-5-10-r84 . 545604 . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Kimura K, Wakamatsu A, Suzuki Y, etal . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 .
• Fiala M, Liu PT, Espinosa-Jeffrey A, etal . Innate immunity and transcription of MGAT-III and Toll-like receptors in Alzheimer's disease patients are improved by bisdemethoxycurcumin. . Proc. Natl. Acad. Sci. U.S.A. . 104 . 31 . 12849–54 . 2007 . 17652175 . 10.1073/pnas.0701267104 . 1937555 . 2007PNAS..10412849F . free .

Notes and References

1. Ihara Y, Nishikawa A, Tohma T, Soejima H, Niikawa N, Taniguchi N . cDNA cloning, expression, and chromosomal localization of human N-acetylglucosaminyltransferase III (GnT-III) . J Biochem . 113 . 6 . 692–8 . Oct 1993 . 10.1093/oxfordjournals.jbchem.a124105 . 8370666 .
2. Web site: Entrez Gene: MGAT3 mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase.