MED8 explained

Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene.^{[1] [2]}

Function

This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Five alternative transcripts encoding four isoforms have been described.^[3]

Interactions

MED8 has been shown to interact with MED26.^[4]

Further reading

• Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R . Composite co-activator ARC mediates chromatin-directed transcriptional activation . Nature . 398 . 6730 . 828–32 . 1999 . 10235267 . 10.1038/19789 . 1999Natur.398..828N . 23646963 .
• Xu XR, Huang J, Xu ZG, Qian BZ, Zhu ZD, Yan Q, Cai T, Zhang X, Xiao HS, Qu J, Liu F, Huang QH, Cheng ZH, Li NG, Du JJ, Hu W, Shen KT, Lu G, Fu G, Zhong M, Xu SH, Gu WY, Huang W, Zhao XT, Hu GX, Gu JR, Chen Z, Han ZG . Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver . Proc. Natl. Acad. Sci. U.S.A. . 98 . 26 . 15089–94 . 2002 . 11752456 . 64988 . 10.1073/pnas.241522398 . 2001PNAS...9815089X . free .
• Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW . Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3 . J. Biol. Chem. . 278 . 17 . 15123–7 . 2003 . 12584197 . 10.1074/jbc.C300054200 . free .
• Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW . A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex . J. Biol. Chem. . 278 . 50 . 49671–4 . 2004 . 14576168 . 10.1074/jbc.C300444200 . free .
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW . A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2 . J. Biol. Chem. . 279 . 7 . 5846–51 . 2004 . 14638676 . 10.1074/jbc.M312523200 . free .
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC . A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology . Mol. Cell . 14 . 5 . 685–91 . 2004 . 15175163 . 10.1016/j.molcel.2004.05.006 . free .
• Léonard VH, Kohl A, Hart TJ, Elliott RM . Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response . J. Virol. . 80 . 19 . 9667–75 . 2006 . 16973571 . 1617248 . 10.1128/JVI.00822-06 .

Notes and References

1. Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC . Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase . Proc Natl Acad Sci U S A . 99 . 16 . 10353–8 . Aug 2002 . 12149480 . 124918 . 10.1073/pnas.162424199 . 2002PNAS...9910353B . free .
2. Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD . Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways . Proc Natl Acad Sci U S A . 95 . 15 . 8538–43 . Aug 1998 . 9671713 . 21111 . 10.1073/pnas.95.15.8538 . 1998PNAS...95.8538J . free .
3. Web site: Entrez Gene: MED8 mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae).
4. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC . A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology . Mol. Cell . 14 . 5 . 685–91 . Jun 2004 . 15175163 . 10.1016/j.molcel.2004.05.006 . free .