MCM3AP explained

80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.^{[1] [2]}

Function

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is an MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.

Interactions

MCM3AP has been shown to interact with:

• MCM3^{[3] [4]} and
• TRIB3.^[5]

Further reading

• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro . DNA Res. . 5 . 1 . 31–9 . 1998 . 9628581 . 10.1093/dnares/5.1.31 . free .
• Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N . A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication . Blood . 95 . 7 . 2321–8 . 2000 . 10733502 . 10.1182/blood.V95.7.2321.
• Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y, Takahashi EI, Sakaguchi N . Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication . Gene . 255 . 2 . 219–27 . 2000 . 11024281 . 10.1016/S0378-1119(00)00336-X .
• Takei Y, Swietlik M, Tanoue A, Tsujimoto G, Kouzarides T, Laskey R . MCM3AP, a novel acetyltransferase that acetylates replication protein MCM3 . EMBO Rep. . 2 . 2 . 119–23 . 2001 . 11258703 . 1083822 . 10.1093/embo-reports/kve026 .
• Kuwahara K, Tomiyasu S, Fujimura S, Nomura K, Xing Y, Nishiyama N, Ogawa M, Imajoh-Ohmi S, Izuta S, Sakaguchi N . Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions . Proc. Natl. Acad. Sci. U.S.A. . 98 . 18 . 10279–83 . 2001 . 11526238 . 56952 . 10.1073/pnas.181335698 . 2001PNAS...9810279K . free .
• EL-Gazzar MA, Maeda K, Nomiyama H, Nakao M, Kuwahara K, Sakaguchi N . PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP . J. Biol. Chem. . 276 . 51 . 48000–8 . 2001 . 11641399 . 10.1074/jbc.M106696200 . free .
• Kono Y, Maeda K, Kuwahara K, Yamamoto H, Miyamoto E, Yonezawa K, Takagi K, Sakaguchi N . MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR . Genes Cells . 7 . 8 . 821–34 . 2002 . 12167160 . 10.1046/j.1365-2443.2002.00562.x . 21946802 . free .
• Takei Y, Assenberg M, Tsujimoto G, Laskey R . The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3 . J. Biol. Chem. . 277 . 45 . 43121–5 . 2002 . 12226073 . 10.1074/jbc.C200442200 . free .
• Nakayama M, Kikuno R, Ohara O . Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs . Genome Res. . 12 . 11 . 1773–84 . 2002 . 12421765 . 187542 . 10.1101/gr.406902 .
• Sakaguchi N, Fujimura S, Kuwahara K . Involvement of GANP in B cell activation in T cell-dependent antigen response . Dev. Immunol. . 9 . 3 . 169–72 . 2002 . 12885157 . 2276099 . 10.1080/1044667031000137647 .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional proteomics mapping of a human signaling pathway . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M . Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins . BMC Genomics . 7 . 155 . 2006 . 16780588 . 1526728 . 10.1186/1471-2164-7-155 . free .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Germinal-center associated nuclear protein (MCM3AP)

Notes and References

1. Takei Y, Tsujimoto G . Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization . J. Biol. Chem. . 273 . 35 . 22177–80 . September 1998 . 9712829 . 10.1074/jbc.273.35.22177 . free .
2. Web site: Entrez Gene: MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein.
3. Takei Y, Assenberg M, Tsujimoto G, Laskey R . The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3 . J. Biol. Chem. . 277 . 45 . 43121–5 . November 2002 . 12226073 . 10.1074/jbc.C200442200 . free .
4. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N . A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication . Blood . 95 . 7 . 2321–8 . April 2000 . 10733502 . 10.1182/blood.V95.7.2321.
5. Zhou Y, Li L, Liu Q, Xing G, Kuai X, Sun J, Yin X, Wang J, Zhang L, He F . E3 ubiquitin ligase SIAH1 mediates ubiquitination and degradation of TRB3 . Cell. Signal. . 20 . 5 . 942–8 . May 2008 . 18276110 . 10.1016/j.cellsig.2008.01.010 .