MARCH2 explained

E3 ubiquitin-protein ligase MARCH2 is an enzyme that in humans is encoded by the MARCH2 gene.^{[1] [2] [3]} It is a member of the MARCH family of E3 ligases, and plays an important role in the turnover of membrane proteins.^[4] MARCH2 has been shown to negatively regulate NF-κB essential modulator function upon viral and bacterial infections.^[5]

Gene name error in Excel

Like the other MARCH and septin genes, care must be exercised when analyzing genetic data containing the MARCH2 gene in Microsoft Excel.^[6] This is due to Excel's autocorrect feature treating the MARCH gene as a date and converting it to a standard date format. The original text cannot be recovered as a result of the conversion. A 2016 study found up to 19.6% of all papers in selected journals to be affected by the gene name error.^[7] The issue can be prevented by using an alias name such as MARCHF2, prepending with an apostrophe ('), or preformatting the cell as text.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Analytical Biochemistry . 236 . 1 . 107–13 . April 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA . Large-scale concatenation cDNA sequencing . Genome Research . 7 . 4 . 353–8 . April 1997 . 9110174 . 139146 . 10.1101/gr.7.4.353 .
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation . Nature Biotechnology . 22 . 6 . 707–16 . June 2004 . 15146197 . 10.1038/nbt971 . 27764390 .
• Nakamura N, Fukuda H, Kato A, Hirose S . MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking . Molecular Biology of the Cell . 16 . 4 . 1696–710 . April 2005 . 15689499 . 1073653 . 10.1091/mbc.E04-03-0216 .
• Fukuda H, Nakamura N, Hirose S . MARCH-III Is a novel component of endosomes with properties similar to those of MARCH-II . Journal of Biochemistry . 139 . 1 . 137–45 . January 2006 . 16428329 . 10.1093/jb/mvj012 .

Notes and References

1. Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z . Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells . Genome Research . 10 . 10 . 1546–60 . October 2000 . 11042152 . 310934 . 10.1101/gr.140200 .
2. Bartee E, Mansouri M, Hovey Nerenberg BT, Gouveia K, Früh K . Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins . Journal of Virology . 78 . 3 . 1109–20 . February 2004 . 14722266 . 321412 . 10.1128/JVI.78.3.1109-1120.2004 .
3. Web site: Entrez Gene: MARCH2 membrane-associated ring finger (C3HC4) 2.
4. Zhang Y, Lu J, Liu X . MARCH2 is upregulated in HIV-1 infection and inhibits HIV-1 production through envelope protein translocation or degradation . Virology . 518 . 293–300 . May 2018 . 29573664 . 10.1016/j.virol.2018.02.003 .
5. 10.15252/embj.2020105139 . Negative regulation of NEMO signaling by the ubiquitin E3 ligase MARCH2 . 2020 . Chathuranga . Kiramage . Kim . Tae-Hwan . Lee . Hyuncheol . Park . Jun-Seol . Kim . Jae-Hoon . Chathuranga . Wijesinghe A Gayan . Ekanayaka . Pathum . Choi . Youn Jung . Lee . Chul-Ho . Kim . Chul-Joong . Jung . Jae U. . Lee . Jong-Soo . The EMBO Journal . 39 . 21 . e105139 . 32935379 . 7604578 . 221745904 .
6. Zeeberg BR, Riss J, Kane DW, Bussey KJ, Uchio E, Linehan WM, Barrett JC, Weinstein JN . 6 . Mistaken identifiers: gene name errors can be introduced inadvertently when using Excel in bioinformatics . BMC Bioinformatics . 5 . 1 . 80 . June 2004 . 15214961 . 459209 . 10.1186/1471-2105-5-80 . free .
7. Ziemann M, Eren Y, El-Osta A . Gene name errors are widespread in the scientific literature . Genome Biology . 17 . 1 . 177 . August 2016 . 27552985 . 4994289 . 10.1186/s13059-016-1044-7 . free .