MAGI1 explained

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 is an enzyme that in humans is encoded by the MAGI1 gene.^{[1] [2] [3]}

Function

The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell–cell contact. The product of this gene may play a role as scaffolding protein at cell–cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified.

Interactions

MAGI1 has been shown to interact with:

• ACCN3,^[4]
• ATN1,^[5]
• Actinin alpha 4,^[6]
• Beta-catenin,^[7]
• Brain-specific angiogenesis inhibitor 1,
• Calcium-activated potassium channel subunit alpha-1,^[8]
• FCHSD2,^[9]
• LRP2,^[10] and
• SYNPO.^[6]

Further reading

• Pirozzi G, McConnell SJ, Uveges AJ, Carter JM, Sparks AB, Kay BK, Fowlkes DM . Identification of novel human WW domain-containing proteins by cloning of ligand targets . The Journal of Biological Chemistry . 272 . 23 . 14611–6 . Jun 1997 . 9169421 . 10.1074/jbc.272.23.14611 . free .
• Dobrosotskaya I, Guy RK, James GL . MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains . The Journal of Biological Chemistry . 272 . 50 . 31589–97 . Dec 1997 . 9395497 . 10.1074/jbc.272.50.31589 . free .
• Wood JD, Yuan J, Margolis RL, Colomer V, Duan K, Kushi J, Kaminsky Z, Kleiderlein JJ, Sharp AH, Ross CA . Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins . Molecular and Cellular Neurosciences . 11 . 3 . 149–60 . Jun 1998 . 9647693 . 10.1006/mcne.1998.0677 . 20003277 . free .
• Dobrosotskaya IY, James GL . MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures . Biochemical and Biophysical Research Communications . 270 . 3 . 903–9 . Apr 2000 . 10772923 . 10.1006/bbrc.2000.2471 .
• Patrie KM, Drescher AJ, Goyal M, Wiggins RC, Margolis B . The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes . Journal of the American Society of Nephrology . 12 . 4 . 667–77 . Apr 2001 . 10.1681/ASN.V124667 . 11274227 . free .
• Dobrosotskaya IY . Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1 . Biochemical and Biophysical Research Communications . 283 . 4 . 969–75 . May 2001 . 11350080 . 10.1006/bbrc.2001.4880 .
• Laura RP, Ross S, Koeppen H, Lasky LA . MAGI-1: a widely expressed, alternatively spliced tight junction protein . Experimental Cell Research . 275 . 2 . 155–70 . May 2002 . 11969287 . 10.1006/excr.2002.5475 .
• Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B . Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1 . The Journal of Biological Chemistry . 277 . 33 . 30183–90 . Aug 2002 . 12042308 . 10.1074/jbc.M203072200 . free.
• Ohno H, Hirabayashi S, Kansaku A, Yao I, Tajima M, Nishimura W, Ohnishi H, Mashima H, Fujita T, Omata M, Hata Y . Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains . Oncogene . 22 . 52 . 8422–31 . Nov 2003 . 14627983 . 10.1038/sj.onc.1206996 . 6289557 .
• Hruska-Hageman AM, Benson CJ, Leonard AS, Price MP, Welsh MJ . PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current . The Journal of Biological Chemistry . 279 . 45 . 46962–8 . Nov 2004 . 15317815 . 10.1074/jbc.M405874200 . free .
• Wright GJ, Leslie JD, Ariza-McNaughton L, Lewis J . Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development . Development . 131 . 22 . 5659–69 . Nov 2004 . 15509766 . 10.1242/dev.01417 . free .
• Benzinger A, Muster N, Koch HB, Yates JR, Hermeking H . Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer . Molecular & Cellular Proteomics . 4 . 6 . 785–95 . Jun 2005 . 15778465 . 10.1074/mcp.M500021-MCP200 . free .
• Mizuhara E, Nakatani T, Minaki Y, Sakamoto Y, Ono Y, Takai Y . MAGI1 recruits Dll1 to cadherin-based adherens junctions and stabilizes it on the cell surface . The Journal of Biological Chemistry . 280 . 28 . 26499–507 . Jul 2005 . 15908431 . 10.1074/jbc.M500375200 . free .
• Patrie KM . Identification and characterization of a novel tight junction-associated family of proteins that interacts with a WW domain of MAGI-1 . Biochimica et Biophysica Acta (BBA) - Molecular Cell Research . 1745 . 1 . 131–44 . Aug 2005 . 16019084 . 10.1016/j.bbamcr.2005.05.011 .
• Bratt A, Birot O, Sinha I, Veitonmäki N, Aase K, Ernkvist M, Holmgren L . Angiomotin regulates endothelial cell-cell junctions and cell motility . The Journal of Biological Chemistry . 280 . 41 . 34859–69 . Oct 2005 . 16043488 . 10.1074/jbc.M503915200 . free .
• Sakurai A, Fukuhara S, Yamagishi A, Sako K, Kamioka Y, Masuda M, Nakaoka Y, Mochizuki N . MAGI-1 is required for Rap1 activation upon cell-cell contact and for enhancement of vascular endothelial cadherin-mediated cell adhesion . Molecular Biology of the Cell . 17 . 2 . 966–76 . Feb 2006 . 16339077 . 1356604 . 10.1091/mbc.E05-07-0647 .

Notes and References

1. Shiratsuchi T, Futamura M, Oda K, Nishimori H, Nakamura Y, Tokino T . Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1 . Biochemical and Biophysical Research Communications . 247 . 3 . 597–604 . Jun 1998 . 9647739 . 10.1006/bbrc.1998.8603 .
2. Margolis RL, Abraham MR, Gatchell SB, Li SH, Kidwai AS, Breschel TS, Stine OC, Callahan C, McInnis MG, Ross CA . cDNAs with long CAG trinucleotide repeats from human brain . Human Genetics . 100 . 1 . 114–22 . Jul 1997 . 9225980 . 10.1007/s004390050476 . 25999127 .
3. Web site: Entrez Gene: MAGI1 membrane associated guanylate kinase, WW and PDZ domain containing 1.
4. Hruska-Hageman AM, Benson CJ, Leonard AS, Price MP, Welsh MJ . PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current . The Journal of Biological Chemistry . 279 . 45 . 46962–8 . Nov 2004 . 15317815 . 10.1074/jbc.M405874200 . free .
5. Wood JD, Yuan J, Margolis RL, Colomer V, Duan K, Kushi J, Kaminsky Z, Kleiderlein JJ, Sharp AH, Ross CA . Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins . Molecular and Cellular Neurosciences . 11 . 3 . 149–60 . Jun 1998 . 9647693 . 10.1006/mcne.1998.0677 . 20003277 . free .
6. Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B . Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1 . The Journal of Biological Chemistry . 277 . 33 . 30183–90 . Aug 2002 . 12042308 . 10.1074/jbc.M203072200 . free.
7. Dobrosotskaya IY, James GL . MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures . Biochemical and Biophysical Research Communications . 270 . 3 . 903–9 . Apr 2000 . 10772923 . 10.1006/bbrc.2000.2471 .
8. Ridgway LD, Kim EY, Dryer SE . MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface . American Journal of Physiology. Cell Physiology . 297 . 1 . C55–65 . Jul 2009 . 19403801 . 3774261 . 10.1152/ajpcell.00073.2009 .
9. Ohno H, Hirabayashi S, Kansaku A, Yao I, Tajima M, Nishimura W, Ohnishi H, Mashima H, Fujita T, Omata M, Hata Y . Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains . Oncogene . 22 . 52 . 8422–31 . Nov 2003 . 14627983 . 10.1038/sj.onc.1206996 . 6289557 .
10. Patrie KM, Drescher AJ, Goyal M, Wiggins RC, Margolis B . The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes . Journal of the American Society of Nephrology . 12 . 4 . 667–77 . Apr 2001 . 10.1681/ASN.V124667 . 11274227 . free .