Lucas Andrew Staehelin Explained

Lucas Andrew Staehelin
Birth Date:10 February 1939
Birth Place:Sydney, Australia
Citizenship:Switzerland-America
Known For:Cell biology, photosynthesis, electron microscopy
Alma Mater:Swiss Federal Institute of Technology
Professor emeritus
Workplaces:University of Colorado Boulder

Lucas Andrew Staehelin (February 10, 1939 – September 28, 2022) was a retired Swiss-American cell biologist.[1] He was professor emeritus at the University of Colorado Boulder.[2]

He developed cryofixation methods and pioneered their use for preserving cellular structures for electron microscope studies.[3] [4] Application of these methods to the analysis of plant, animal and bacterial cells brought insights into the nanoscale architecture and functional organization of membranous organelles and cytoskeletal systems.[5] [6] [7] [8] Staehelin taught undergraduate and graduate courses in cellular and molecular biology at the University of Colorado Boulder.

Early life and education

Staehelin was born in Sydney, Australia on February 10, 1939. In 1948 he moved to Switzerland where he attended the Gymnasium in Bern. He studied natural sciences as an undergraduate at the Swiss Federal Institute of Technology, Zürich from 1959 to 1963, where he earned his doctorate in plant cell biology in 1966, studying in the laboratory of Kurt Mühlethaler.[9] From 1966 to 1969 he worked as a scientist at the Physics and Engineering Laboratory of the Department of Scientific and Industrial Research in New Zealand. He was a postdoctoral fellow from 1969 to 1970 at Harvard University.[10]

Career

Staehelin became an assistant professor in the Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, in 1970, was promoted to associate professor in 1973, to full professor in 1978, and to professor emeritus in 2006. He held visiting professorships at Albert-Ludwigs University (1978), Swiss Federal Institute of Technology (1984, 1992), and University of Melbourne (1998).

He has authored and co-authored more than 300 scientific publications (H-Index 78).[11] [12]

Research

Staehelin's research focused on using cryofixation methods to produce improved images of cellular structures by means of freeze-fracture, thin section and immunolabeling electron microscopy, and by electron tomography techniques. His studies of photosynthetic membranes led to the characterization of differences in structure and function of grana and stroma thylakoid membranes,[13] of chlorosomes,[14] and the attachment of plastoglobules to thylakoids.[15] Vitrification of plant cells using propane-jet freezing led to the discovery that cellulose fibrils were synthesized by plasma membrane-associated rosette particle complexes,[16] and the demonstration that vesicle-mediated secretion and membrane recycling in turgid cells involves characteristic, transient membrane configurations.[17] High pressure freezing of intact plant tissues led to new models of plant cytokinesis, first as seen in thin section images[18] [19] and then by electron tomography. These studies led to the discovery of the cell plate assembly matrix within which the assembly of the cell plate from vesicles occurs by previously unknown mechanisms.[20] Electron tomography analysis enabled his group to produce nanoscale models of endoplasmic reticulum, Golgi apparatus and trans Golgi network membranes[21] [22] [23] [24] The Golgi matrix/scaffold was shown to originate on COPII vesicles and to capture passing Golgi to mediate ER-Golgi vesicle transfer as postulated by the stop-pluck-and-go hypothesis of Golgi trafficking.[25]

Awards

Notes and References

  1. Web site: 2022-10-05 . L. Andrew Staehelin, CU Boulder molecular biologist, dies at 83 . 2023-05-14 . Colorado Arts and Sciences Magazine . en.
  2. Web site: 2016-09-22 . Andrew Staehelin . 2022-03-23 . Molecular, Cellular & Developmental Biology . en.
  3. Gilkey . John C. . Staehelin . L. Andrew . 1986 . Advances in ultrarapid freezing for the preservation of cellular ultrastructure . Journal of Electron Microscopy Technique . en . 3 . 2 . 177–210 . 10.1002/jemt.1060030206 . 1553-0817.
  4. Cheung . Alice Y . Cosgrove . Daniel J . Hara-Nishimura . Ikuko . Jürgens . Gerd . Lloyd . Clive . Robinson . David G . Staehelin . L Andrew . Weijers . Dolf . 2021-09-15 . A rich and bountiful harvest: key discoveries in plant cell biology . The Plant Cell . 34 . 53–71 . 10.1093/plcell/koab234 . 34524464 . 8773953 . 1040-4651.
  5. Nevo . Reinat . Charuvi . Dana . Tsabari . Onie . Reich . Ziv . April 2012 . Composition, architecture and dynamics of the photosynthetic apparatus in higher plants: The photosynthetic apparatus in higher plants . The Plant Journal . en . 70 . 1 . 157–176 . 10.1111/j.1365-313X.2011.04876.x. 22449050 . free .
  6. Kirchhoff . Helmut . 2019-03-08 . Chloroplast ultrastructure in plants . New Phytologist . 223 . 2 . 565–574 . 10.1111/nph.15730 . 30721547 . 206221619 . 0028-646X. free .
  7. Jürgens . Gerd . Park . Misoon . Richter . Sandra . Touihri . Sonja . Krause . Cornelia . El Kasmi . Farid . Mayer . Ulrike . 2015-01-26 . Plant cytokinesis: a tale of membrane traffic and fusion . Biochemical Society Transactions . 43 . 1 . 73–78 . 10.1042/BST20140246 . 25619248 . 0300-5127.
  8. Ogbu . Chinemerem P. . Roy . Sourav . Vecchio . Alex J. . January 2022 . Disruption of Claudin-Made Tight Junction Barriers by Clostridium perfringens Enterotoxin: Insights from Structural Biology . Cells . en . 11 . 5 . 903 . 10.3390/cells11050903 . 2073-4409 . 8909277 . 35269525. free .
  9. Web site: Chemistry Tree - L. Andrew Staehelin .
  10. Web site: L. Andrew Staehelin . 22 March 2022 . ResearchGate.
  11. Web site: L. Andrew Staehelin, University of Colorado Boulder, Department of Molecular, Cellular, and Developmental Biology . 2021-12-17 . ResearchGate.
  12. Web site: Web of Science . 2022-01-26 . www.webofscience.com.
  13. Staehelin . L. Andrew . 2003-04-01 . Chloroplast structure: from chlorophyll granules to supra-molecular architecture of thylakoid membranes . Photosynthesis Research . en . 76 . 1 . 185–96 . 10.1023/A:1024994525586 . 16228577 . 5022848 . 1573-5079.
  14. Staehelin . L. Andrew . Golecki . Jochen R. . Drews . Gerhart . 1980-04-01 . Supramolecular organization of chlorosomes (chlorobium vesicles) and of their membrane attachment sites in Chlorobium Limicola . Biochimica et Biophysica Acta (BBA) - Bioenergetics . en . 589 . 1 . 30–45 . 10.1016/0005-2728(80)90130-9 . 7356977 . 0005-2728.
  15. Rottet . Sarah . Besagni . Céline . Kessler . Felix . 2015-09-01 . The role of plastoglobules in thylakoid lipid remodeling during plant development . Biochimica et Biophysica Acta (BBA) - Bioenergetics . SI: Chloroplast Biogenesis . en . 1847 . 9 . 889–99 . 10.1016/j.bbabio.2015.02.002 . 25667966 . 0005-2728. free .
  16. Giddings . T H Jr . Brower . D L . Staehelin . L A . 1980-02-01 . Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls. . Journal of Cell Biology . 84 . 2 . 327–339 . 10.1083/jcb.84.2.327 . 0021-9525 . 2110545 . 7189756.
  17. Staehelin . L. A. . Chapman . R. L. . 1987-05-01 . Secretion and membrane recycling in plant cells: novel intermediary structures visualized in ultrarapidly frozen sycamore and carrot suspension-culture cells . Planta . en . 171 . 1 . 43–57 . 10.1007/BF00395066 . 24227269 . 1987Plant.171...43S . 29633957 . 1432-2048.
  18. Samuels . A L . Giddings . T H Jr . Staehelin . L A . 1995-09-15 . Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants. . Journal of Cell Biology . 130 . 6 . 1345–1357 . 10.1083/jcb.130.6.1345 . 0021-9525 . 2120572 . 7559757.
  19. Otegui . Marisa . Staehelin . L. Andrew . 2000-06-01 . Syncytial-Type Cell Plates: A Novel Kind of Cell Plate Involved in Endosperm Cellularization of Arabidopsis . The Plant Cell . 12 . 6 . 933–947 . 10.1105/tpc.12.6.933 . 1040-4651 . 149094 . 10852938.
  20. Otegui . M. S. . Mastronarde . D. N. . Kang . B. H. . Bednarek . S. Y. . Staehelin . L. A. . September 2001 . Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography . The Plant Cell . 13 . 9 . 2033–2051 . 10.1105/tpc.13.9.2033 . 1040-4651 . 139450 . 11549762 . 3871426 .
  21. ROBINSON . DAVID G. . 2020-05-27 . Plant Golgi ultrastructure . Journal of Microscopy . 280 . 2 . 111–121 . 10.1111/jmi.12899 . 32420623 . 218680885 . 0022-2720.
  22. Staehelin . L. Andrew . Kang . Byung-Ho . 2008-08-01 . Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography . Plant Physiology . 147 . 4 . 1454–1468 . 10.1104/pp.108.120618 . 0032-0889 . 2492626 . 18678738.
  23. Kang . Byung-Ho . Nielsen . Erik . Preuss . Mary Lai . Mastronarde . David . Staehelin . L. Andrew . 2011 . Electron Tomography of RabA4b- and PI-4Kβ1-Labeled Trans Golgi Network Compartments in Arabidopsis . Traffic . en . 12 . 3 . 313–329 . 10.1111/j.1600-0854.2010.01146.x . 21134079 . 13965499 . 1600-0854. free .
  24. Donohoe . Bryon S. . Kang . Byung-Ho . Gerl . Mathias J. . Gergely . Zachary R. . McMichael . Colleen M. . Bednarek . Sebastian Y. . Staehelin . L. Andrew . 2013 . Cis-Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae . Traffic . en . 14 . 5 . 551–567 . 10.1111/tra.12052 . 1600-0854 . 3622843 . 23369235.
  25. Nebenführ . Andreas . Gallagher . Larry A. . Dunahay . Terri G. . Frohlick . Jennifer A. . Mazurkiewicz . Anna M. . Meehl . Janet B. . Staehelin . L. Andrew . 1999-12-01 . Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System1 . Plant Physiology . 121 . 4 . 1127–1141 . 10.1104/pp.121.4.1127 . 0032-0889 . 59480 . 10594100.
  26. Web site: Singleview - Alexander von Humboldt-Stiftung .
  27. Web site: List of Members .
  28. Web site: Historic Fellows . 2021-12-17 . American Association for the Advancement of Science . en.
  29. Web site: 2007-07-25 . CU-Boulder's Andrew Staehelin Named First Ever Fellow Of American Society Of Plant Biologists . 2021-12-17 . CU Boulder Today . en.

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