| Lochloosa Creek | |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | Florida |
| Subdivision Type3: | County |
| Subdivision Name3: | Alachua County |
| Source1 Location: | Saluda Swamp |
| Source1 Coordinates: | 29.6656°N -82.1597°W |
| Mouth Location: | Lochloosa Lake |
| Mouth Elevation: | 56feet |
| Mouth Coordinates: | 29.5483°N -82.1378°W |
| Basin Size: | 42.7sqmi |
| Custom Label: | GNIS |
Lochloosa Creek is a stream that flows from north to south through eastern Alachua County, Florida.[1] Its watershed composed of through natural and Silvicultural land use. It is the largest tributary to Lochloosa Lake. Lochloosa Creek is longest creek in Alachua County.
Forming from the Saluda Swamp, Lochloosa Creek begins as a small trickle of a stream that flows south. It flows through agricultural lands.