This article contains a list of the most studied restriction enzymes whose names start with E to F inclusive. It contains approximately 110 enzymes.
| Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers |
| EacI | | Eubacterium acidaminophilum | 5' GGATC
3' CCTAG | 5' ---GGATCNNNN {{pad|2em}} N--- 3'
3' ---CCTAGNNNNN {{pad|2em}} --- 5' | | AclWI, AlwI, BinI, | BpuFI, | BspPI, | |
| BstH9I, | Bst31TI, Bth617I, Ral8I | |
|
| EaeI | | Enterobacter aerogenes | 5' YGGCCR
3' RCCGGY | 5' ---Y {{pad|2em}} GGCCR--- 3'
3' ---RCCGG {{pad|2em}} Y--- 5' | |
| Eae46I | | Enterobacter aerogenes 46 | 5' CCGCGG
3' GGCGCC | 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5' | |
| EaeAI | | Enterobacter aerogenes | 5' CCCGGG
3' GGGCCC | 5' ---C {{pad|2em}} CCGGG--- 3'
3' ---GGGCC {{pad|2em}} C--- 5' | AhyI, Cfr9I, EclRI, PaeBI, PspAI, PspALI, XcyI, XmaI, XmaCI |
| EagI | | Enterobacter agglomerans | 5' CGGCCG
3' GCCGGC | 5' ---C {{pad|2em}} GGCCG--- 3'
3' ---GCCGG {{pad|2em}} C--- 5' | AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIII |
| EagBI | | Enterobacter agglomerans CBNU45 | 5' CGATCG
3' GCTAGC | 5' ---CGAT {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} TAGC--- 5' | Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorII |
| EagMI | | Enterobacter agglomerans M3 | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI |
| Eam1104I | | Enterobacter amnigenus RFL1104 | 5' CTCTTC
3' GAGAAG | 5' ---CTCTTCN {{pad|2em}} NNN--- 3'
3' ---GAGAAGNNNN {{pad|2em}} --- 5' | |
| Eam1105I | | Enterobacter amnigenus RFL1105 | 5' GACN<sub>5</sub>GTC
3' CTGN<sub>5</sub>CAG | 5' ---GACNNN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NNNCAG--- 5' | | AhdI, AspEI, | BmeRI, | BspOVI, | - | DriI, EclHKI, NruGI | |
|
| EarI | | Enterobacter aerogenes | 5' CTCTTC
3' GAGAAG | 5' ---CTCTTCN {{pad|2em}} NNN--- 3'
3' ---GAGAAGNNNN {{pad|2em}} --- 5' | |
| EcaI | | Enterobacter cloacae | 5' GGTNACC
3' CCANTGG | 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5' | AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I |
| EciI | | Escherichia coli | 5' GGCGGA
3' CCGCCT | 5' ---GGCGGAN<sub>8</sub>NNN {{pad|2em}} --- 3'
3' ---CCGCCTN<sub>8</sub>N {{pad|2em}} NN--- 5' | |
| Eci125I | | Escherichia coli VKM-125 | 5' GGTNACC
3' CCANTGG | 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5' | AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I |
| EclI | | Enterobacter cloacae | 5' CAGCTG
3' GTCGAC | 5' ---CAG {{pad|2em}} CTG--- 3'
3' ---GTC {{pad|2em}} GAC--- 5' | |
| Ecl136II | | Enterobacter cloacae RFL136 | 5' GAGCTC
3' CTCGAG | 5' ---GAG {{pad|2em}} CTC--- 3'
3' ---CTC {{pad|2em}} GAG--- 5' | |
| EclHKI | | Enterobacter cloacae | 5' GACN<sub>5</sub>GTC
3' CTGN<sub>5</sub>CAG | 5' ---GACNNN {{pad|2em}} NNGTC--- 3'
3' ---CTGNN {{pad|2em}} NNNCAG--- 5' | | AhdI, AspEI, | BmeRI, | BspOVI, | - | DriI, Eam1105I, NruGI | |
|
| EclRI | | Enterobacter cloacae R | 5' CCCGGG
3' GGGCCC | 5' ---C {{pad|2em}} CCGGG--- 3'
3' ---GGGCC {{pad|2em}} C--- 5' | AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI, XcyI, XmaI, XmaCI |
| EclXI | | Enterobacter cloacae 590 | 5' CGGCCG
3' GCCGGC | 5' ---C {{pad|2em}} GGCCG--- 3'
3' ---GCCGG {{pad|2em}} C--- 5' | AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIII |
| Ecl18kI | 2FQZ | Enterobacter cloacae 18k | 5' CCNGG
3' GGNCC | 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5' | |
| Ecl37kI | | Enterobacter cloacae 37k | 5' CTGCAG
3' GACGTC | 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' | AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenI |
| Ecl2zI | | Enterobacter cloacae 2z | 5' CTGCAG
3' GACGTC | 5' ---CTGCA {{pad|2em}} G--- 3'
3' ---G {{pad|2em}} ACGTC--- 5' | AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenI |
| Eco24I | | Escherichia coli RFL24 | 5' GRGCYC
3' CYCGRG | 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5' | |
| Eco31I | | Escherichia coli RFL31 | 5' GGTCTC
3' CCAGAG | 5' ---GGTCTCN {{pad|2em}} NNNN--- 3'
3' ---CCAGAGNNNNN {{pad|2em}} --- 5' | |
| Eco32I | | Escherichia coli RFL32 | 5' GATATC
3' CTATAG | 5' ---GAT {{pad|2em}} ATC--- 3'
3' ---CTA {{pad|2em}} TAG--- 5' | |
| Eco47I | | Escherichia coli RFL47 | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI |
| Eco47III | | Escherichia coli RFL47 | 5' AGCGCT
3' TCGCGA | 5' ---AGC {{pad|2em}} GCT--- 3'
3' ---TCG {{pad|2em}} CGA--- 5' | AfeI, AitI, Aor51H, FunI |
| Eco52I | | Escherichia coli RFL52 | 5' CGGCCG
3' GCCGGC | 5' ---C {{pad|2em}} GGCCG--- 3'
3' ---GCCGG {{pad|2em}} C--- 5' | AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIII |
| Eco56I | | Escherichia coli RFL56 | 5' GCCGGC
3' CGGCCG | 5' ---G {{pad|2em}} CCGGC--- 3'
3' ---CGGCC {{pad|2em}} G--- 5' | |
| Eco57I | | Escherichia coli RFL57 | 5' CTGAAG
3' GACTTC | 5' ---CTGAAGN<sub>12</sub>NNNN {{pad|2em}} --- 3'
3' ---GACTTCN<sub>12</sub>NN {{pad|2em}} NN--- 5' | AcuI, BspKT5I |
| Eco64I | | Escherichia coli RFL64 | 5' GGYRCC
3' CCRYGG | 5' ---G {{pad|2em}} GYRCC--- 3'
3' ---CCRYG {{pad|2em}} G--- 5' | BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAI |
| Eco72I | | Escherichia coli RFL72 | 5' CACGTG
3' GTGCAC | 5' ---CAC {{pad|2em}} GTG--- 3'
3' ---GTG {{pad|2em}} CAC--- 5' | AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCI |
| Eco78I | | Escherichia coli RFL78 | 5' GGCGCC
3' CCGCGG | 5' ---GGC {{pad|2em}} GCC--- 3'
3' ---CCG {{pad|2em}} CGG--- 5' | |
| Eco81I | | Escherichia coli RFL81 | 5' CCTNAGG
3' GGANTCC | 5' ---CC {{pad|2em}} TNAGG--- 3'
3' ---GGANT {{pad|2em}} CC--- 5' | AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI |
| Eco88I | | Escherichia coli RFL88 | 5' CYCGRG
3' GRGCYC | 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5' | AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAI |
| Eco91I | | Escherichia coli RFL91 | 5' GGTNACC
3' CCANTGG | 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5' | Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAII |
| Eco105I | | Escherichia coli RFL105 | 5' TACGTA
3' ATGCAT | 5' ---TAC {{pad|2em}} GTA--- 3'
3' ---ATG {{pad|2em}} CAT--- 5' | |
| Eco130I | | Escherichia coli RFL130 | 5' CCWWGG
3' GGWWCC | 5' ---C {{pad|2em}} CWWGG--- 3'
3' ---GGWWC {{pad|2em}} C--- 5' | |
| Eco147I | | Escherichia coli RFL147 | 5' AGGCCT
3' TCCGGA | 5' ---AGG {{pad|2em}} CCT--- 3'
3' ---TCC {{pad|2em}} GGA--- 5' | AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI |
| Eco255I | | Escherichia coli RFL255 | 5' AGTACT
3' TCATGA | 5' ---AGT {{pad|2em}} ACT--- 3'
3' ---TCA {{pad|2em}} TGA--- 5' | | Acc113I, AssI, | BmcAI, Bpa34I, | - | DpaI, RflFII, ScaI, ZrmI | |
|
| Eco1831I | | Escherichia coli RFL1831 | 5' CCSGG
3' GGSCC | 5' --- {{pad|2em}} CCSGG--- 3'
3' ---GGSCC {{pad|2em}} --- 5' | | AhaI, AseII, AsuC2I, BcnI, | - | BpuMI, | CauII, EcoHI, HgiS22I | |
|
| EcoA4I | | Escherichia coli A4 | 5' GGTCTC
3' CCAGAG | 5' ---GGTCTCN {{pad|2em}} NNNN--- 3'
3' ---CCAGAGNNNNN {{pad|2em}} --- 5' | |
| EcoHI | | Escherichia coli HI | 5' CCSGG
3' GGSCC | 5' --- {{pad|2em}} CCSGG--- 3'
3' ---GGSCC {{pad|2em}} --- 5' | AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22I, Kpn49kII, NciI |
| EcoHK31I | | Escherichia coli HK31 | 5' YGGCCR
3' RCCGGY | 5' ---Y {{pad|2em}} GGCCR--- 3'
3' ---RCCGG {{pad|2em}} Y--- 5' | |
| EcoICRI | | Escherichia coli 2bT | 5' GAGCTC
3' CTCGAG | 5' ---GAG {{pad|2em}} CTC--- 3'
3' ---CTC {{pad|2em}} GAG--- 5' | |
| Eco75KI | | Escherichia coli BKM | 5' GRGCYC
3' CYCGRG | 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5' | |
| Eco57MI | | Escherichia coli RFL57M | 5' CTGRAG
3' GACYTC | 5' ---CTGRAGN<sub>12</sub>NNNN {{pad|2em}} --- 3'
3' ---GACYTCN<sub>12</sub>NN {{pad|2em}} NN--- 5' | |
| EcoNI | | Escherichia coli | 5' CCTN<sub>5</sub>AGG
3' GGAN<sub>5</sub>TCC | 5' ---CCTNN {{pad|2em}} NNNAGG--- 3'
3' ---GGANNN {{pad|2em}} NNTCC--- 5' | |
| EcoO44I | | Escherichia coli O44 Hiromi | 5' GGTCTC
3' CCAGAG | 5' ---GGTCTCN {{pad|2em}} NNNN--- 3'
3' ---CCAGAGNNNNN {{pad|2em}} --- 5' | |
| EcoO65I | | Escherichia coli K11a | 5' GGTNACC
3' CCANTGG | 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5' | AcrII, Bse64I, BstEII, BstPI, BstT9I, EcaI, Eco91I, EcoO128I |
| EcoO109I | 1WTD | Escherichia coli H709c | 5' RGGNCCY
3' YCCNGGR | 5' ---RG {{pad|2em}} GNCCY--- 3'
3' ---YCCNG {{pad|2em}} GR--- 5' | |
| EcoO128I | | Escherichia coli O128Ly3 | 5' GGTNACC
3' CCANTGG | 5' ---G {{pad|2em}} GTNACC--- 3'
3' ---CCANTG {{pad|2em}} G--- 5' | AspAI, Bse64I, BstEII, BstT9I, EcaI, EcoO65I, NspSAII, PspEI |
| EcoP15I | | Escherichia coli P15 | 5' CAGCAG
3' GTCGTC | 5' ---CAGCAGN<sub>25</sub>NN {{pad|2em}} --- 3'
3' ---GTCGTCN<sub>25</sub> {{pad|2em}} NN--- 5' | |
| EcoR124II | , Search | Escherichia coli | 5' 5'-GAAN<sub>7</sub>RTCG-3'
| 5' {{pad|2em}}
{{pad|2em}} | |
| EcoRI | 1QC9, Search EcoR1 structures | Escherichia coli RY13 | 5' GAATTC
3' CTTAAG | 5' ---G {{pad|2em}} AATTC--- 3'
3' ---CTTAA {{pad|2em}} G--- 5' | |
| EcoRII | 1NA6 | Escherichia coli R245 | 5' CCWGG
3' GGWCC | 5' --- {{pad|2em}} CCWGG--- 3'
3' ---GGWCC {{pad|2em}} --- 5' | ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI, ZanI |
| EcoRV | 1RVE Search EcoRV | Escherichia coli J62 pLG74 | 5' GATATC
3' CTATAG | 5' ---GAT {{pad|2em}} ATC--- 3'
3' ---CTA {{pad|2em}} TAG--- 5' | |
| EcoT14I | | Escherichia coli TB14 | 5' CCWWGG
3' GGWWCC | 5' ---C {{pad|2em}} CWWGG--- 3'
3' ---GGWWC {{pad|2em}} C--- 5' | |
| EcoT22I | | Escherichia coli TB22 | 5' ATGCAT
3' TACGTA | 5' ---ATGCA {{pad|2em}} T--- 3'
3' ---T {{pad|2em}} ACGTA--- 5' | BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5II |
| EcoT38I | | Escherichia coli TH38 | 5' GRGCYC
3' CYCGRG | 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5' | |
| EcoVIII | | Escherichia coli E1585-68 | 5' AAGCTT
3' TTCGAA | 5' ---A {{pad|2em}} AGCTT--- 3'
3' ---TTCGA {{pad|2em}} A--- 5' | |
| Eco13kI | | Escherichia coli 13k | 5' CCNGG
3' GGNCC | 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5' | |
| Eco21kI | | Escherichia coli 21k | 5' CCNGG
3' GGNCC | 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5' | |
| Eco27kI | | Escherichia coli 27 | 5' CYCGRG
3' GRGCYC | 5' ---C {{pad|2em}} YCGRG--- 3'
3' ---GRGCY {{pad|2em}} C--- 5' | Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAI |
| Eco29kI | | Escherichia coli | 5' CCGCGG
3' GGCGCC | 5' ---CCGC {{pad|2em}} GG--- 3'
3' ---GG {{pad|2em}} CGCC--- 5' | |
| Eco53kI | | Escherichia coli 53k | 5' GAGCTC
3' CTCGAG | 5' ---GAG {{pad|2em}} CTC--- 3'
3' ---CTC {{pad|2em}} GAG--- 5' | |
| Eco137kI | | Escherichia coli 137k | 5' CCNGG
3' GGNCC | 5' --- {{pad|2em}} CCNGG--- 3'
3' ---GGNCC {{pad|2em}} --- 5' | |
| EgeI | | Enterobacter gergoviae NA | 5' GGCGCC
3' CCGCGG | 5' ---GGC {{pad|2em}} GCC--- 3'
3' ---CCG {{pad|2em}} CGG--- 5' | |
| EheI | | Erwinia herbicola 9/5 | 5' GGCGCC
3' CCGCGG | 5' ---GGC {{pad|2em}} GCC--- 3'
3' ---CCG {{pad|2em}} CGG--- 5' | |
| ErhI | | Erwinia rhaponici B9 | 5' CCWWGG
3' GGWWCC | 5' ---C {{pad|2em}} CWWGG--- 3'
3' ---GGWWC {{pad|2em}} C--- 5' | |
| ErhB9I | | Erwinia rhaponici B9 | 5' CGATCG
3' GCTAGC | 5' ---CGAT {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} TAGC--- 5' | Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorII |
| ErhB9II | | Erwinia rhaponici B9 | 5' CCWWGG
3' GGWWCC | 5' ---C {{pad|2em}} CWWGG--- 3'
3' ---GGWWC {{pad|2em}} C--- 5' | |
| ErpI | | Erwinia rhaponici | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI |
| EsaBC3I | | Environmental sample BC3 | 5' TCGA
3' AGCT | 5' ---TC {{pad|2em}} GA--- 3'
3' ---AG {{pad|2em}} CT--- 5' | |
| EsaBC4I | | Environmental sample BC4 | 5' GGCC
3' CCGG | 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5' | |
| EspI | | Eucapsis sp. | 5' GCTNAGC
3' CGANTCG | 5' ---GC {{pad|2em}} TNAGC--- 3'
3' ---CGANT {{pad|2em}} CG--- 5' | |
| Esp3I | | Erwinia sp. RFL3 | 5' CGTCTC
3' GCAGAG | 5' ---CGTCTCN {{pad|2em}} NNNN--- 3'
3' ---GCAGAGNNNNN {{pad|2em}} --- 5' | |
| Esp4I | | Erwinia sp. RFL4 | 5' CTTAAG
3' GAATTC | 5' ---C {{pad|2em}} TTAAG--- 3'
3' ---GAATT {{pad|2em}} C--- 5' | |
| Esp1396I | | Enterobacter sp. RFL1396 | 5' CCAN<sub>5</sub>TGG
3' GGTN<sub>5</sub>ACC | 5' ---CCANNNN {{pad|2em}} NTGG--- 3'
3' ---GGTN {{pad|2em}} NNNNACC--- 5' | AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I | |
| Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers |
| FalI | | Flavobacterium aquatile Ob10 | 5' AAGN<sub>5</sub>CTT
3' TTCN<sub>5</sub>GAA | 5' ---AAGN<sub>5</sub>CTTN<sub>7</sub>NNNNNN {{pad|2em}} --- 3'
3' ---TTCN<sub>5</sub>GAAN<sub>7</sub>N {{pad|2em}} NNNNN--- 5' | |
| FalII | | Flavobacterium aquatile Ob10 | 5' CGCG
3' GCGC | 5' ---CG {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} GC--- 5' | BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI, ThaI |
| FaqI | | Flavobacterium aquatile RFL1 | 5' GGGAC
3' CCCTG | 5' ---GGGACN<sub>8</sub>NN {{pad|2em}} NNNN--- 3'
3' ---CCCTGN<sub>8</sub>NNNNNN {{pad|2em}} --- 5' | |
| FatI | | Flavobacterium aquatile NL3 | 5' CATG
3' GTAC | 5' --- {{pad|2em}} CATG--- 3'
3' ---GTAC {{pad|2em}} --- 5' | |
| FauI | | Flavobacterium aquatile | 5' CCCGC
3' GGGCG | 5' ---CCCGCNNNN {{pad|2em}} NN--- 3'
3' ---GGGCGNNNNNN {{pad|2em}} --- 5' | |
| FauBII | | Flavobacterium aureus B | 5' CGCG
3' GCGC | 5' ---CG {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} GC--- 5' | Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI, ThaI |
| FauNDI | | Flavobacterium aquatile ND | 5' CATATG
3' GTATAC | 5' ---CA {{pad|2em}} TATG--- 3'
3' ---GTAT {{pad|2em}} AC--- 5' | |
| FbaI | | Flavobacterium balustinum | 5' TGATCA
3' ACTAGT | 5' ---T {{pad|2em}} GATCA--- 3'
3' ---ACTAG {{pad|2em}} T--- 5' | | AbaI, BclI, BsiQI, | - | BspXII, BstT7I, Ksp22I, | ParI | |
|
| FblI | | Flavobacterium balustinum | 5' GTMKAC
3' CAKMTG | 5' ---GT {{pad|2em}} MKAC--- 3'
3' ---CAKM {{pad|2em}} TG--- 5' | AccI, XmiI |
| FbrI | | Flavobacterium breve | 5' GCNGC
3' CGNCG | 5' ---GC {{pad|2em}} NGC--- 3'
3' ---CGN {{pad|2em}} CG--- 5' | |
| FdiI | | Fremyella diplosiphon | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BthAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI |
| FdiII | | Fremyella diplosiphon | 5' TGCGCA
3' ACGCGT | 5' ---TGC {{pad|2em}} GCA--- 3'
3' ---ACG {{pad|2em}} CGT--- 5' | Acc16I, AosI, AviII, FspI, MstI, NsbI, PamI, Pun14627I |
| FgoI | | Fervidobacterium gondwanense AB39T | 5' CTAG
3' GATC | 5' ---C {{pad|2em}} TAG--- 3'
3' ---GAT {{pad|2em}} C--- 5' | |
| FmuI | | Flavobacterium multivorum | 5' GGNCC
3' CCNGG | 5' ---GGNC {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} CNGG--- 5' | FmuI AvcI, BavAII, Bce22I, Bsu54I, NspIV, Pde12I, UnbI |
| FnuAI | | Fusobacterium nucleatum A | 5' GANTC
3' CTNAG | 5' ---G {{pad|2em}} ANTC--- 3'
3' ---CTNA {{pad|2em}} G--- 5' | |
| FnuCI | | Fusobacterium nucleatum C | 5' GATC
3' CTAG | 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' | Bme12I, Bsp67I, Bst19II, CcyI, FnuEI, MgoI, NphI, SauMI |
| FnuDI | | Fusobacterium nucleatum D | 5' GGCC
3' CCGG | 5' ---GG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GG--- 5' | |
| FnuDII | | Fusobacterium nucleatum D | 5' CGCG
3' GCGC | 5' ---CG {{pad|2em}} CG--- 3'
3' ---GC {{pad|2em}} GC--- 5' | Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FnuDII, ThaI |
| FnuDIII | | Fusobacterium nucleatum D | 5' GCGC
3' CGCG | 5' ---GCG {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} GCG--- 5' | AspLEI, BspLAI, BstHHI, CfoI, HhaI, Hin6I, HinP1I, HspAI, SciNI |
| FnuEI | | Fusobacterium nucleatum E | 5' GATC
3' CTAG | 5' --- {{pad|2em}} GATC--- 3'
3' ---CTAG {{pad|2em}} --- 5' | Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, Sau3AI |
| Fnu4HI | | Fusobacterium nucleatum 4H | 5' GCNGC
3' CGNCG | 5' ---GC {{pad|2em}} NGC--- 3'
3' ---CGN {{pad|2em}} CG--- 5' | |
| FokI | 2FOK | Flavobacterium okeanokoites | 5' GGATG
3' CCTAC | 5' ---GGATGN<sub>8</sub>N {{pad|2em}} NNNN--- 3'
3' ---CCTACN<sub>8</sub>NNNNN {{pad|2em}} --- 5' | |
| FriOI | | Flavobacterium sp. O9 | 5' GRGCYC
3' CYCGRG | 5' ---GRGCY {{pad|2em}} C--- 3'
3' ---C {{pad|2em}} YCGRG--- 5' | |
| FseI | | Frankia sp. Eu11b | 5' GGCCGGCC
3' CCGGCCGG | 5' ---GGCCGG {{pad|2em}} CC--- 3'
3' ---CC {{pad|2em}} GGCCGG--- 5' | |
| FsiI | | Frankia sp. | 5' RAATTY
3' YTTAAR | 5' ---R {{pad|2em}} AATTY--- 3'
3' ---YTTAA {{pad|2em}} R--- 5' | |
| FspI | | Fischerella sp. | 5' TGCGCA
3' ACGCGT | 5' ---TGC {{pad|2em}} GCA--- 3'
3' ---ACG {{pad|2em}} CGT--- 5' | Acc16I, AosI, AviII, FdiII, MstI, NsbI, PamI, Pun14627I |
| FspII | | Fischerella sp. | 5' TTCGAA
3' AAGCTT | 5' ---TT {{pad|2em}} CGAA--- 3'
3' ---AAGC {{pad|2em}} TT--- 5' | Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, Ssp1I, SviI |
| Fsp1604I | | Flavobacterium sp. I 16-04 | 5' CCWGG
3' GGWCC | 5' ---CC {{pad|2em}} WGG--- 3'
3' ---GGW {{pad|2em}} CC--- 5' | ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, SleI |
| FspAI | | Flexibacter sp. TV-m21K | 5' RTGCGCAY
3' YACGCGTR | 5' ---RTGC {{pad|2em}} GCAY--- 3'
3' ---YACG {{pad|2em}} CGTR--- 5' | |
| FspBI | | Flavobacterium sp. RFLI | 5' CTAG
3' GATC | 5' ---C {{pad|2em}} TAG--- 3'
3' ---GAT {{pad|2em}} C--- 5' | |
| Fsp4HI | | Flavobacterium sp. 4H | 5' GCNGC
3' CGNCG | 5' ---GC {{pad|2em}} NGC--- 3'
3' ---CGN {{pad|2em}} CG--- 5' | |
| FspMSI | | Fischerella sp. | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BthAI, DsaIV, ErpI, HgiBI, HgiEI, Kzo49I, SmuEI |
| FssI | | Fibrobacter succinogenes S85 | 5' GGWCC
3' CCWGG | 5' ---G {{pad|2em}} GWCC--- 3'
3' ---CCWG {{pad|2em}} G--- 5' | Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Kzo49I, VpaK11AI |
| FunI | | Fischerella uniformis | 5' AGCGCT
3' TCGCGA | 5' ---AGC {{pad|2em}} GCT--- 3'
3' ---TCG {{pad|2em}} CGA--- 5' | AfeI, AitI, Aor51H, Eco47III |
| FunII | | Fischerella uniformis | 5' GAATTC
3' CTTAAG | 5' ---G {{pad|2em}} AATTC--- 3'
3' ---CTTAA {{pad|2em}} G--- 5' | | |
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