List of restriction enzyme cutting sites explained

See main article: restriction enzyme.

A restriction enzyme or restriction endonuclease is a special type of biological macromolecule that functions as part of the "immune system" in bacteria. One special kind of restriction enzymes is the class of "homing endonucleases", these being present in all three domains of life, although their function seems to be very different from one domain to another.

The classical restriction enzymes cut up, and hence render harmless, any unknown (non-cellular) DNA that enters a bacterial cell as a result of a viral infection. They recognize a specific DNA sequence, usually short (3 to 8 bp), and cut it, producing either blunt or overhung ends, either at or nearby the recognition site.

Restriction enzymes are quite variable in the short DNA sequences they recognize. An organism often has several different enzymes, each specific to a distinct short DNA sequence.^[1]

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Restriction enzymes catalog

The list includes some of the most studied examples of restriction endoncleases. The following information is given:The whole list contains more than 1,200 enzymes, but databases register about 4,000.^[2] To make a list that is accessible to navigation, this list has been divided into different pages. Each page contains somewhere between 120-150 entries. Choose a letter to go to a specific part of the list:

See also

• List of homing endonuclease cutting sites
• Restriction enzyme.
• Isoschizomer.
• Detailed articles about certain restriction enzymes: EcoRI, HindIII, BglII.
• Homing endonuclease.
• Introns and inteins.
• Intragenomic conflict: Homing endonuclease genes.
• I-CreI homing endonuclease.

External links

Databases and lists of restriction enzymes:

• Very comprehensive database of restriction enzymes supported by New England Biolabs. It includes all kind of biological, structural, kinetical and commercial information about thousands of enzymes. Also includes related literature for every molecule: Web site: REBASE . Roberts RJ, Vincze T, Posfai J, Macelis D . 2010-05-23.
• Detailed information for biochemical experiments: Web site: Enzyme finder . 2010-01-07 . https://web.archive.org/web/20100108142840/http://www.neb.com/nebecomm/EnzymeFinder.asp . 2010-01-08 . dead . New England Biolabs.
• Alphabetical list of enzymes and their restriction sites: Web site: GenScript Restriction Enzyme webpage . 2010-01-07 . https://web.archive.org/web/20090704062431/http://www.genscript.com/cgi-bin/products/enzyme.cgi?op=all_ez . 2009-07-04 . dead .
• General information about restriction sites and biochemical conditions for restriction reactions: Web site: Restriction Enzymes Resource . Promega . 2010-01-07 . https://web.archive.org/web/20020203123020/http://www.promega.com/guides/re_guide/ . 2002-02-03 . dead .

Databases of proteins:

• Database of structures of proteins, solved at atomic resolution: Web site: Worldwide Protein Data Bank. . 2010-01-25.
• Databases of proteins: Web site: UniProtKB/Swiss-Prot & TrEMBL . Swiss Institute of Bioinformatics (SIB) . European Bioinformatics Institute (EBI) . Swiss Institute of Bioinformatics . European Bioinformatics Institute . amp . Swiss-Prot is a curated protein sequence database which strives to provide a high level of annotation (such as the description of the function of a protein, its domains structure, post-translational modifications, variants, etc.), a minimal level of redundancy and high level of integration with other databases. TrEMBL is a computer-annotated supplement of Swiss-Prot that contains all the translations of EMBL nucleotide sequence entries not yet integrated in Swiss-Prot. . 2010-01-25.

Notes and References

1. Roberts RJ . Restriction and modification enzymes and their recognition sequences . Nucleic Acids Res. . 8 . 1 . r63–r80 . January 1980 . 6243774 . 327257 . 10.1093/nar/8.1.197-d .
2. Web site: REBASE . Roberts RJ, Vincze T, Posfai J, Macelis D . Restriction Enzyme Database. . 2010-05-23.