| Trail name | Length | Location | Original Rail Road |
|---|
| Auburndale TECO Trail | | Polk County | Florida Western and Northern Railroad (Seaboard Air Line Railroad) |
| Blackwater Heritage State Trail | 9.6miles | Santa Rosa County | Florida and Alabama Railroad (Bagdad Land and Lumber Company)[1] |
| Blountstown Greenway Bike Path | 3.4miles | Calhoun County | Marianna and Blountstown Railroad[2] |
| Boca Grande Bike Path | 6.5miles | Gasparilla Island and Boca Grande, Florida | Charlotte Harbor and Northern Railway (Seaboard Air Line Railroad) |
| Cady Way Trail | 6.5miles | Orange County | East Florida and Atlantic Railroad (Seaboard Air Line Railroad) |
| Cape Haze Pioneer Trail | 11.5miles | Charlotte County | Charlotte Harbor and Northern Railway (Seaboard Air Line Railroad) |
| Chain of Lakes Trail | 4.7miles | Polk County | South Florida Railroad--Bartow Branch (Atlantic Coast Line Railroad) |
| Cross Seminole Trail | 23miles | Seminole County | Sanford and Indian River Railroad (Atlantic Coast Line Railroad) |
| Cross Town Trail | | Citrus County | Silver Springs, Ocala and Gulf Railroad (Atlantic Coast Line Railroad) |
| East Central Regional Rail Trail | | Volusia and Brevard Counties | Atlantic Coast, St. Johns and Indian River Railroad (Florida East Coast Railroad)[3] |
| Florida Keys Overseas Heritage Trail | 106miles | Monroe County | Overseas Railroad (Florida East Coast Railroad) |
| Fort Fraser Trail | 7.75miles | Polk County | Lakeland—Fort Myers Line (Atlantic Coast Line Railroad) |
| Fountain Lake Trail | | Lake County | Leesburg and Indian River Railroad and Tavares, Orlando and Atlantic Railroad (Seaboard Air Line Railroad) |
| Four Freedoms Trail | 12miles | Madison County | Florida Midland and Georgia Railroad (Valdosta Southern Railroad) |
| Gainesville-Hawthorne Trail State Park | 16miles | | Florida Southern Railway (Atlantic Coast Line Railroad) |
| General James A. Van Fleet State Trail | 29.2miles | Polk, Lake, and Sumter Counties | Florida Western and Northern Railroad (Seaboard Air Line Railroad) |
| Good Neighbor Trail | 10.7miles | | Florida Southern Railway -- Brooksville Branch (Atlantic Coast Line Railroad) |
| Hardy Trail | 1miles | Pasco County | Florida Central and Peninsular Railroad (Seaboard Air Line Railroad) |
| Heritage Trail | | Suwannee County | Live Oak, Tampa and Charlotte Harbor Railroad (Atlantic Coast Line Railroad)[4] [5] |
| Jacksonville-Baldwin Rail Trail | 26miles | Duval County | Jacksonville and Southwestern Railroad (Atlantic Coast Line Railroad) |
| John Yarbrough Linear Park | 6miles | Lee County | Lakeland—Fort Myers Line (Atlantic Coast Line Railroad) |
| Lake Denham Trail | | Sumter and Lake Counties | Atlantic Coast Line Railroad -- High Springs—Croom Line |
| Legacy Trail | 18.5miles | Sarasota County | Florida West Shore Railway (Seaboard Air Line Railroad) |
| Monticello Bike Trail | 3.1miles | Jefferson County | Perry Cutoff (Atlantic Coast Line Railroad)[6] |
| Nature Coast State Trail | 31.7miles | Dixie, Gilchrist, and Levy Counties | Jacksonville and Southwestern Railroad extension and Perry Cutoff (Atlantic Coast Line Railroad) |
| O'Leno to Ichetucknee Trail | | Columbia County | Live Oak, Tampa and Charlotte Harbor Railroad (Atlantic Coast Line Railroad)[7] [8] |
| Palatka-Lake Butler State Trail | | Union, Bradford, Clay, and Putnam Counties | Georgia Southern and Florida Railway (Southern Railway) |
| Palatka-to-St. Augustine State Trail | 19miles | Putnam and St. Johns Counties | Florida East Coast Railroad -- Palatka Branch[9] |
| Pinellas Trail | 45miles | Pinellas County |
|
| Punta Gorda Linear Park | 1miles | Charlotte County | Florida Southern Railway (Atlantic Coast Line Railroad) |
| Seminole-Wekiva Trail | 3.3miles | Seminole County | Orange Belt Railway (Atlantic Coast Line Railroad) |
| South Dade Rail Trail | 20.5miles | Miami-Dade County | Florida East Coast Railway |
| South Lake Trail | 13miles | Lake County | Orange Belt Railway (Atlantic Coast Line Railroad) |
| Suwannee River Greenway | 12.5miles | Suwannee County | Live Oak, Tampa and Charlotte Harbor Railroad (Atlantic Coast Line Railroad)[10] |
| Tallahassee-St. Marks Historic Railroad State Trail | 16miles | Leon and Wakulla Counties | Tallahassee Railroad (Seaboard Air Line Railroad) |
| Tav Lee Trail | | Leesburg to Eustis | St. Johns and Lake Eustis Railway (Atlantic Coast Line Railroad) |
| Upper Tampa Bay Trail | 7.25miles | Hillsborough County | Tampa and Gulf Coast Railroad-Tarpon Springs Branch (Seaboard Air Line Railroad) |
| West Orange Trail | 22miles | Orange County | Orange Belt Railway (Atlantic Coast Line Railroad) |
| Withlacoochee State Trail | 46miles | Pasco, Hernando, and Citrus Counties | Silver Springs, Ocala and Gulf Railroad and Florida Southern Railway (Atlantic Coast Line Railroad) | |
