This is a list of the Spanish PROMUSICAE Top 20 Singles number-ones of 2000.[1]
| Issue Date | Song | Artist |
|---|---|---|
| 1 January | Enrique Iglesias | |
| 8 January | ||
| 15 January | ||
| 22 January | ||
| 29 January | Bunbury | |
| 5 February | Ace of Base | |
| 12 February | Christina Aguilera | |
| 19 February | Aqua | |
| 26 February | Oasis | |
| 4 March | Bloodhound Gang | |
| 11 March | ||
| 18 March | Madonna | |
| 25 March | ||
| 1 April | Sobreviviré | Mónica Naranjo |
| 8 April | ||
| 15 April | ||
| 22 April | Agustito (Ketama Goes to Miami Remix) | Ketama |
| 29 April | Sobreviviré | Mónica Naranjo |
| 6 May | Gloria Estefan | |
| 13 May | ||
| 20 May | Bon Jovi | |
| 27 May | Britney Spears | |
| 3 June | Sex Machine (Get Up I Feel Like Being Like A Sex Machine) | Tony Sweat |
| 10 June | ||
| 17 June | ||
| 24 June | ||
| 1 July | ||
| 8 July | It's My Life | Bon Jovi |
| 15 July | José el Francés | |
| 22 July | If You Leave Me Now | Mónica Naranjo |
| 29 July | ||
| 5 August | Boom Boom | |
| 12 August | ||
| 19 August | ||
| 26 August | Ya no quiero tu querer | José el Francés |
| 2 September | ||
| 9 September | Madonna | |
| 16 September | ||
| 23 September | ||
| 30 September | ||
| 7 October | ||
| 14 October | Modjo | |
| 21 October | ||
| 28 October | U2 | |
| 4 November | ||
| 11 November | ||
| 18 November | ||
| 25 November | No cambié | Tamara |
| 2 December | ||
| 9 December | ||
| 16 December | ||
| 23 December | ||
| 30 December |