Clubs are not only members of the Federación Ecuatoriana de Fútbol, Ecuador's football governing body, but of one of 18 provincial football associations in the country. Clubs play in either Primera Categoría (divided into Serie A or Serie B) or Segunda Categoría.
| Club | Home city | Stadium | Aggregate position last season | Founded | |
|---|---|---|---|---|---|
| Aucas | Quito | Gonzalo Pozo Ripalda | 1st | 1945 | |
| Barcelona | Guayaquil | Monumental Banco Pichincha | 5th | 1925 | |
| Cumbayá | Quito | Olímpico Atahualpa | 14th | 1970 | |
| Delfín | Manta | Estadio Jocay | 8th | 1989 | |
| Deportivo Cuenca | Cuenca | Alejandro Serrano Aguilar | 7th | 1981 | |
| El Nacional | Quito | Olímpico Atahualpa | Serie B | 1964 | |
| Emelec | Guayaquil | George Capwell | 6th | 1929 | |
| Gualaceo | Azogues | Jorge Andrade Cantos | 11th | 2000 | |
| Guayaquil City | Guayaquil | Christian Benítez Betancourt | 10th | 2007 | |
| Independiente del Valle | Quito | Banco Guayaquil | 3rd | 1958 | |
| LDU Quito | Quito | Rodrigo Paz Delgado | 4th | 1918 | |
| Libertad | Loja | Reina del Cisne | Serie B | 2017 | |
| Mushuc Runa | Ambato | COAC Mushuc Runa | 13th | 2003 | |
| Orense | Machala | 9 de Mayo | 9th | 2009 | |
| Técnico Universitario | Ambato | Bellavista Universidad Indoamérica | 12th | 1971 | |
| Universidad Católica | Quito | Olímpico Atahualpa | 2nd | 1963 |
| Club | Home city | Stadium | Finishing position last season | Founded | |
|---|---|---|---|---|---|
| 9 de Octubre | Guayaquil | Modelo Alberto Spencer Herrera | Serie A | 1912 | |
| América de Quito | Quito | Olímpico Atahualpa | 4th | 1939 | |
| Búhos ULVR | Guayaquil | Modelo Alberto Spencer Herrera | 7th | 1899 | |
| Chacaritas | Pelileo | Ciudad de Pelileo | 6th | 1960 | |
| Cuniburo | Cayambe | Olímpico Guillermo Albornoz | Segunda Categoría | 1992 | |
| Imbabura | Ibarra | Olímpico Ciudad de Ibarra | 5th | 1993 | |
| Independiente Juniors | Sangolquí | Banco Guayaquil | 2nd | 2017 | |
| Macará | Guayaquil | Estadio Bellavista | Serie A | 1939 | |
| Manta | Manta | Estadio Jocay | 8th | 1998 | |
| Vargas Torres | Esmeraldas | Folke Anderson | Segunda Categoría | 1983 |
See main article: Segunda Categoría.
| Season | Club | Opponent | Score | |
|---|---|---|---|---|
| 2009 | LDU Quito |  Fluminense | 5–4 | |
| 2019 | Independiente del Valle |  Colón | 3–1 | |
| 2022 | Independiente del Valle | São Paulo | 2–0 |
| Season | Club | Opponent | Aggregate score | |
|---|---|---|---|---|
| 2009 | LDU Quito | Internacional | 4–0 | |
| 2010 | LDU Quito | Estudiantes | 2–1 | |
| 2023 | Independiente del Valle | Flamengo | 1–1 (5–4 p) |