This is a list of cities in Guinea-Bissau order by population. All settlements with a population of over 5,000 are included.
| Cities in Guinea-Bissau | |||||
|---|---|---|---|---|---|
| Rank | City | Population (2009 Census)[1] | Region | ||
| align=right | 1 | 387,909 | Bissau | ||
| align=right | 2 | 43,556 | Gabú | ||
| align=right | 3 | 29,556 | Bafatá | ||
| align=right | 4 | 12,044 | Cacheu | ||
| align=right | 5 | 9,898 | Oio | ||
| align=right | 6 | 9,005 | Oio | ||
| align=right | 7 | 8,313 | Oio | ||
| align=right | 8 | 7,898 | Quinara | ||
| align=right | 9 | 5,891 | Cacheu | ||
| align=right | 10 | 5,825 | Biombo | ||
| align=right | 11 | 5,454 | Quinara | ||
| align=right | 12 | 5,081 | Tombali | ||
| align=right | 13 | 5,026 | Bolama | ||