| Post: | Ambassador |
| Body: | the United States to the Czech Republic |
| Insignia: | US Department of State official seal.svg |
| Insigniasize: | 120 |
| Insigniacaption: | Seal of the United States Department of State |
| Incumbent: | Bijan Sabet |
| Incumbentsince: | February 15, 2023 |
| Nominator: | The President of the United States |
| Appointer: | The President |
| Appointer Qualified: | with Senate advice and consent |
| Inaugural: | Adrian A. Basora as Ambassador Extraordinary and Plenipotentiary |
| Formation: | June 15, 1992 |
| Website: | U.S. Embassy - Prague |
The diplomatic post of United States ambassador to the Czech Republic was created after the dissolution of Czechoslovakia and the United States recognizing the new nation of the Czech Republic on January 1, 1993.
In June 1992, the Slovak parliament voted to declare sovereignty and the Czech-Slovak federation dissolved peacefully on January 1, 1993. The United States recognized the Czech Republic and Slovakia as independent nations and moved to establish diplomatic relations. The previous ambassador to Czechoslovakia, Adrian A. Basora, continued as the ambassador to the Czech Republic and Theodore Russell, who served as deputy chief of mission under Ambassador Shirley Temple Black, became the first U.S. ambassador to Slovakia later that year.
| Ambassador Extraordinary and Plenipotentiary | June 15, 1992 | July 20, 1992 | July 15, 1995 | |||
| June 27, 1995 | August 31, 1995 | August 31, 1998 | ||||
| October 22, 1998 | December 10, 1998 | December 16, 2000 | ||||
| August 7, 2001 | August 28, 2001 | December 16, 2003 | ||||
| October 6, 2003 | January 13, 2004 | September 15, 2006 | ||||
| September 14, 2006 | October 12, 2006 | January 20, 2009 | ||||
| Mary Thompson-Jones | Chargés d’affaires ad interim | January 20, 2009 | Unknown | May 2010 | ||
| John Ordway | May 2010 | Unknown | August 2010 | |||
| Joseph Pennington | August 2010 | Unknown | December 30, 2010 | |||
| Ambassador Extraordinary and Plenipotentiary | December 30, 2010 | January 28, 2011 | August 12, 2014 | |||
| July 23, 2014 | September 30, 2014 | January 20, 2017 | ||||
| Steve King[1] | October 18, 2017 | December 6, 2017 | January 20, 2021 | |||
| Jennifer Bachus | Chargés d’affaires ad interim | January 20, 2021 | March 29, 2022 | |||
| Michael Dodman | March 29, 2022 | August 18, 2022 | ||||
| Christy Agor | August 18, 2022 | January 31, 2023 | ||||
| Bijan Sabet | Ambassador Extraordinary and Plenipotentiary | December 13, 2022 | February 15, 2023 | Incumbent |